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Chemical reagents, kits, columns, and other products used for DNA synthesis, and to subsequently run purification columns; includes genetic assembly systems.
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AMPK activator 12 is a potent AMP-activated protein kinase (AMPK) activator and GDF15 inducer. It increases GDF15 protein levels in human hepatic cells.
Potent AMPK activator
GDF15 inducer
Increases GDF15 protein levels in human hepatic cells
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An activator of EIF2AK1/HRI; activates HRI and induces eIF2α phosphorylation in a surrogate cell-based reporter assay; selectively inhibits the proliferation of CRL-2813 cells over NIH3T3 fibroblasts (IC50s = 0.46 and 3.4 µM, respectively); selectively inhibits the proliferation of CRL-2813 cells transiently transfected with non-targeted siRNA over CRL-2813 cells transiently transfected with HRI-targeted siRNA (IC50s = 0.66 and 5 µM, respectively); increases mRNA expression and protein levels of the eIF2α target CHOP in CRL-2813 cells at 5 µM
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Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryopreservation method yields high cell viability post-thaw and provides the convenience of immediately dispensing healthy reporter cells into assay plates. There is no need for intermediate preparatory steps such as the spin-and-rinse of cells, viability determinations, or cell titer adjustments prior to assay setup.
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DMT-2′Fluoro-dU Phosphoramidite is a uridine phosphoramidite featuring a fluorine atom at the 2' position. This compound is suitable for nucleoside modification and the synthesis of DNA. Fluorination, a prevalent chemical derivatization in pharmaceutical chemistry, leverages fluorine's unique biophysical and biochemical properties, including its small size and high electronegativity. The ongoing research and development of novel fluorinated nucleic acid molecules are crucial for advancements in drug discovery and fundamental biochemical studies.
Uridine phosphoramidite with fluorine at the 2' position
Can be used for nucleoside modification
Suitable for synthesis of DNA
Supports innovative drug discovery
Aids basic biochemical studies
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This kit detects GM2A in human serum plasma urine tissue homogenates and cell lysates with a broad detection range of 15 6 pg/mL to 1000 pg/mL offering high sensitivity down to 3 9 pg/mL Utilizing a sandwich ELISA technique it enables quantitative analysis of GM2A levels within 1-5 hours of incubation time The assay requires a sample volume of 50-100 L and measures absorbance at 450 nm for accurate detection Designed for flexibility it accommodates diverse biological matrices while maintaining robust performance making it suitable for research applications requiring precise measurement of GM2A concentrations in multiple sample types
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The Biotage Custom PhyTip 500 μL is a specialized pipette tip column designed for high-throughput biomolecule purification utilizing proprietary Dual Flow Chromatography technology. This product offers custom resin specifications for personalized purification applications and is compatible with various automated liquid handling systems, enhancing laboratory workflows.
Column type: PhyTip
Purification technology: Dual Flow Chromatography
Application: Biomolecule purification, custom
Total column volume: 500 μL
Resin volume: 160 μL
Resin type: Custom packed
Automation compatibility: Yes
Sample concentration: High due to small elution volumes
Reproducibility: High purity and yield
Process gentleness: Gentle purification for active proteins
Pack size (typical): 96 columns
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