Nucleic Acid Introduction Reagents
Cell Applications Inc Cytofect™ Neuron Transfection Kit: 25 x 24-Well Rxns
Cytofect™ Neuron Transfection Kit: 25 x 24-Well Rxns
Expression Systems Expres2 TR (Transfection Reagent), 75 ul (included in BestBac kits)
In collaboration with Expres2ion Biotechnologies, Expression Systems offers Expres2 TR Transfection Reagent, formulated and optimized for insect cell culture. Expres2 TR is a cationic lipid formulation designed for the co-transfection or transfection step in baculovirus vector production.
• Formulation works seamlessly with Transfection Medium
• Optimized for use in insect cell culture
• Consistently high transfection rates Increases virus vector production and transient expression
• In stock for immediate shipment
To download Instructions for Use and/or COA please visit: www.expressionsystems.com
SignaGen Laboratories PolyJet™ In Vitro DNA Transfection Reagent
PolyJet DNA In Vitro Tranfection Reagent was formulated by our proprietary biodegradable polymer synthesis technology to ensure very efficient gene delivery to commonly used mammalian cell lines and a broad ranges of hard-to-transfect mammalian cells with minimal toxicity.
Nova Biostorage 0.75ml 2D Data-Matrix Coded Screw Cap Tubes, Internal Thread V-Bottom, Precapped Light Blue Screw Caps, Micronic 96-2 Rack, High Cover, Barcoded A1-H1 Side, Gamma Irradiated, 40 Racks
0.75ml 2D Data-Matrix coded Screw Cap tubes with Internal Thread V-bottom. Precapped with Light Blue color screw caps in Micronic 96-2 Rack and high cover. (Barcoded A1-H1 side)
Gamma Irradiated
Working volume at 21°C: 0.48ml
Quantity = 40 Racks
HORIZON DISCOVERY BIOSCIENCES LIMITED DharmaFECT 1 Transfection Reagent | 5x1.5ml
The most broadly applicable DharmaFECT formulation for optimal siRNA or microRNA transfection into a wide range of cell types for successful RNAi experiments. DharmaFECT 1 has been validated in over 35 cell types.
Lonza Walkersville Human B Cell Nucleofector Kit
The Nucleofector 2b Device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary human B cells.The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human B Cells0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Kyfora Bio DMG-PEG2000 500 MG
DMG-PEG 2000 (1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000) is a PEGylated lipid used in the formation of lipid nanoparticles (LNP) for the development of mRNA vaccines such as COVID-19 vaccine and for the delivery of nucleic acids such as RNAis. It is derived from dimyristoyl glycerol (DMG) which behaves similar to phospholipids, namely in the way they associate with membranes. PEGylation of the lipid enhances bioavailability and prevents opsonization of the LNP. This product is for research use only. Key application(s): mRNA vaccine, gene delivery
New England Biolabs, Inc. Universal miRNA Cloning Linker – 5 µg
Linker/RNA Ligation Protocol: The Universal miRNA cloning linker has a 3' blocking group (amine) to prevent self ligation, circularization and ligation to RNA at the 5' end. It also contains the BanI restriction site compatible with the miRNA cloning protocol used by Bartel. For ligation, resuspend the lyophilized oligonucleotide in RNAse-free water to 100 M stock concentration. (A resuspension volume of 8.6 µl will give a 0.58ug/l or 100 µM stock solution). Ligations are carried out with T4 RNA Ligase I in the absence of ATP. Approximately 1 ug is typically used per reaction.
ATCC CF52.Tr (CRL-6244) Canine Adherent Fibroblast Cell Line, BSL-1
CF52.Tr (CRL-6244) is a canine fibroblast adherent cell line from ATCC's NBL Collection supplied for in vitro research applications. The line is maintained under standard mammalian culture conditions and handled at biosafety level 1.
- Derived from Canis familiaris (dog).
- Fibroblast cell type with adherent growth properties.
- Recommended medium: DMEM with 4 mM L-glutamine and 10% fetal bovine serum.
- Incubation: 37°C in a 5% CO2 atmosphere.
- Biosafety level 1 handling.
- Part of the NBL Collection and fully accessioned for research use.
Sigma Aldrich Fine Chemicals Biosciences NeuroPorterTM Transfection
Neuroporter(TM) Transfection Reagent is a unique formulation of a proprietary cationic lipid optimized for delivery of DNA into primary neurons glial cells and cultured neuronal cell lines with high efficiency and low toxicity. The Neuroporter(TM) Transfection Kit was designed for difficult-to-transfect primary neurons addressing past problems such as poor cell viability low transfection efficiency and neuro-degeneration.
Amsbio LLC GenePORTER Transfection reagent
The GenePORTER Reagent is the original transfection lipid developed by Genlantis containing a unique formulation of the neutral lipid dioleoyl phosphatidylethanolamine (DOPE) and using the proprietary and innovative Direct Hydrophilic Conjugation (DHC) technology. The GenePORTER reagent is optimized for serum-free transfection conditions in a wide variety of commonly used cells. The GenePORTER reagent is especially effective in Jurkat cells and gives the highest transfection efficiencies relative to all other commercial reagents.