Chemical Transformation Kits
Chemical Transformation Kits
Thermo Scientific™ TransformAid Bacterial Transformation Kit
Kit for quick and convenient preparation of competent E.coli cells for guaranted transformation efficiencies of more than 107 transformants per μg of plasmid DNA.
MP Biomedicals™ Alkali-Cation™ Yeast Transformation Kit
Provides all of the reagents required to perform a quick and easy transformation. Alkali-Cation™ Yeast Transformation Kit is easy to use, robust, and its scalability makes it great for the high-efficiency transformation needed in two hours.
MP Biomedicals™ EZ-Yeast™ Transformation Kit
Designed for high-throughput transformation with efficiencies up to 103 transformants per μg plasmid DNA. Perfect for a quick analysis of potentially positive two-hybrid colonies, the EZ-Yeast™ Transformation Kit does not require fully competent cells prior to transformation.
Multi-Copy Pichia Expression Kit
The Multi-Copy Pichia Expression Kit contains the pPIC3.5K, pPIC9K, and pAO815 vectors for production and selection of Pichia strains that contain more than one copy of the gene of interest.
Invitrogen™ EasySelect™ Pichia Expression Kit
The EasySelect™ Pichia Expression Kit provides all needed components for protein production in the yeast Pichia pastoris.
Invitrogen™ PichiaPink™ Secretion Optimization Kit
The PichiaPink™ Secretion Optimization Kit is a new recombinant protein expression system based on the yeast Pichia pastoris.
Invitrogen™ Pichia Expression Kit, original kit
The Original Pichia Expression Kit is designed for high-level expression of recombinant protein in the yeast Pichia pastoris.
Invitrogen™ PichiaPink™ Secretion Signal Set
The PichiaPink™ Secretion Signal Set is a set of 8 DNA fragments encoding secretion signal sequences. The PichiaPink™ Secretion Signal Set is designed to be easily ligated in front of your gene.
Amsbio LLC (formerly Genlantis) TurboCells™ BL21Competent Cells
High-level E. coli expression using three-minute transformation protocol
Zymo Research Corporation Frozen-EZ Yeast Transformation II™ Kit (120 Rxns)
Version II further improves the efficiency and reliability ofits predecessor. The competent yeast cells prepared with these reagents can be used immediately for transformation experiments or can be stored frozen at or below -70C for direct use in the future. This method is suitable for both circular and linear plasmid transformations.
New England Biolabs, Inc. Blunt/TA Ligase Master Mix – 250 reactions
Blunt/TA Ligase Master Mix is a ready-to-use solution of T4 DNA Ligase, proprietary ligation enhancer, and optimized reaction buffer. This master mix is specifically formulated to improve ligation and transformation of both blunt-end and single-base overhang substrates. The master mix format simplifies reaction set-up, ensures an optimized ratio of enzyme and buffer components, and yields robust, rapid ligation of all types of DNA ends using a short incubation time at room temperature. No thawing is necessary as it remains liquid during storage at -20C. Ligations for subcloning can be carried out in small volumes with low DNA concentrations, allowing users to conserve precious DNA samples and directly transform many strains of chemically competent E. coli without dilution.
Zymo Research Corporation XJa Autolysis™ Glycerol Stock (1 Tube) (Add 1 ml 500x Arabinose)
Additionally, it does not require use of any potentially interferingcomponents such as detergents, commonly found in various lytic buffers.Highlights Simple and controlled autolysis of E. coli Strains can by lysed in minutes after harvesting Method compatible with most buffer systems Ideal for protein expression and purification, also applicable for extraction of nucleic acids Scale up to lyse more samples without increase in time or labor
Zymo Research Corporation Mix & Go E. coli Transformation Kit & Buffer Set, up to 60 ml
The methods eliminate the requirement of heat-shocking and related procedures. Instead, transformation can be performed by adding DNA to prepared Mix & Go cells and the mixture spread directly to a culture plate. Transformation efficiencies typically range from 108–109 transformants/µg of pUC19 DNA but can vary depending on the strain of E. coli. Most E. coli strains respond well to the Mix & Go preparation method and demonstrate fast transformation kinetics.The procedures are easy. Simply culture the E. coli strain of your choice in ZymoBroth™ medium (or SOB), wash and then resuspend the cells in the provided uniquely formulated buffers. The cells are now ready for transformation.
Agilent Technologies XL1-BLUE MR SUPERCOMP CELLS
Cells; XL1-Blue MR Supercompetent; Stratagene; For cloning w/o F episome, greater-than-equal-to 1 x 109 transformants/microgram; Deficient in all known E. coli K12 restriction systems; Increase efficiency of introducing eukaryotic DNA
Zymo Research Corporation XJb Autolysis Glycerol Stock, 1 ml 500x L-Arabinose, 1 ml
Additionally, it does not require use of any potentially interferingcomponents such as detergents, commonly found in various lytic buffers.Highlights• Simple and controlled autolysis of E. coli• Strains can by lysed in minutes after harvesting• Method compatible with most buffer systems• Ideal for protein expression and purification, also applicable for extraction of nucleic acids• Scale up to lyse more samples without increase in time or labor