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Kit for quick and convenient preparation of competent E.coli cells for guaranted transformation efficiencies of more than 107 transformants per μg of plasmid DNA.
Provides all of the reagents required to perform a quick and easy transformation. Alkali-Cation™ Yeast Transformation Kit is easy to use, robust, and its scalability makes it great for the high-efficiency transformation needed in two hours.
Designed for high-throughput transformation with efficiencies up to 103 transformants per μg plasmid DNA. Perfect for a quick analysis of potentially positive two-hybrid colonies, the EZ-Yeast™ Transformation Kit does not require fully competent cells prior to transformation.
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The methods eliminate the requirement of heat-shocking and related procedures. Instead, transformation can be performed by adding DNA to prepared Mix & Go cells and the mixture spread directly to a culture plate. Transformation efficiencies typically range from 108–109 transformants/µg of pUC19 DNA but can vary depending on the strain of E. coli. Most E. coli strains respond well to the Mix & Go preparation method and demonstrate fast transformation kinetics.The procedures are easy. Simply culture the E. coli strain of your choice in ZymoBroth™ medium (or SOB), wash and then resuspend the cells in the provided uniquely formulated buffers. The cells are now ready for transformation.
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Version II further improves the efficiency and reliability ofits predecessor. The competent yeast cells prepared with these reagents can be used immediately for transformation experiments or can be stored frozen at or below -70C for direct use in the future. This method is suitable for both circular and linear plasmid transformations.
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The Mix & Go! E. coli Transformation Kit and Mix & Go! E. coli Transformation Buffer Set are convenient methods for the preparation of competent E. coli cells for simple and highly efficient DNA transformation. The Mix & Go! method completely eliminates the requirement for heat shocking and related procedures. Instead, Mix & Go! bacterial transformation can be performed by adding DNA to Mix & Go! competent cells and spreading onto a plate. Transformation efficiencies are typically on the order of 108-109 transformants/µg plasmid DNA with most E. coli strains. Uniquely formulated reagents make it easy to generate Mix & Go! competent cells from current E. coli strains that are available in the laboratory. Simply grow the E. coli strain of your choice, wash, then resuspend the cells in the provided buffers. The cells are now transformation ready!
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Cells; XL1-Blue MR Supercompetent; Stratagene; For cloning w/o F episome, greater-than-equal-to 1 x 109 transformants/microgram; Deficient in all known E. coli K12 restriction systems; Increase efficiency of introducing eukaryotic DNA
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