Transfection Reagents
New England Biolabs, Inc. dam-/dcm- Competent E. coli – 6 x 0.2 ml/tube
Methyltransferase deficient chemically competent E. coli cells suitable for growth of plasmids free of Dam and Dcm methylation. Note that dam- strains are not recommended as a host for primary cloning/ligation. The dam mutation can result in an increased mutation rate in the cell and a reduction in the transformation efficiency. DNA should be maintained in a dam+ strain unless there is a specific need for DNA free of Dam or Dcm methylation.
Kyfora Bio DMG-PEG2000 1 GR
DMG-PEG 2000 (1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000) is a PEGylated lipid used in the formation of lipid nanoparticles (LNP) for the development of mRNA vaccines such as COVID-19 vaccine and for the delivery of nucleic acids such as RNAis. It is derived from dimyristoyl glycerol (DMG) which behaves similar to phospholipids, namely in the way they associate with membranes. PEGylation of the lipid enhances bioavailability and prevents opsonization of the LNP. This product is for research use only. Key application(s): mRNA vaccine, gene delivery
Kyfora Bio Transporter 5 Transfection Reagent 50ML
Kyfora Bio's Transporter 5 is a high-quality, ready-to-use transfection reagent derived from our popular PEI MAX (Polyethyleneimine MAX). This versatile reagent is suitable for both stable and transient transfections across a variety of cell lines, including HEK293 and CHO cells. Transporter 5 consistently delivers high yields of viral vectors (AAV and LV), recombinant proteins, and monoclonal antibodies, all while maintaining optimal cell viability. Its scalability and compatibility with a range of cell culture media make it an excellent choice among available transfection reagents on the market.
Kyfora Bio Transporter 5 Transfection Reagent 5ML
Kyfora Bio's Transporter 5 is a high-quality, ready-to-use transfection reagent derived from our popular PEI MAX (Polyethyleneimine MAX). This versatile reagent is suitable for both stable and transient transfections across a variety of cell lines, including HEK293 and CHO cells. Transporter 5 consistently delivers high yields of viral vectors (AAV and LV), recombinant proteins, and monoclonal antibodies, all while maintaining optimal cell viability. Its scalability and compatibility with a range of cell culture media make it an excellent choice among available transfection reagents on the market.
HORIZON DISCOVERY BIOSCIENCES LIMITED SIGLO GREEN TRANSFECTION IND
NC1381293 SIGLO GREEN TRANSFECTION IND
Boca Scientific Inc ViroMag R/L Viral Gene Delivery Reagent for Retrovirus/Lentivirus, 1ml
ViroMag R/L Transduction Reagent is a magnetic nanoparticles formulation optimized for Retroviruses and Lentiviruses. Based on Magnetofection technology, this reagent allows concentration of the complete applied dose of Retro/Lentiviral particles onto cells within minutes, inducing a significant improvement of virus infectivity with extremely low vector doses. Due to its specific properties, ViroMag R/L is ideal for infecting non-permissive cells. It demonstrates an exceptionally high efficiency in promoting, controlling and assisting viral transductions. No molecular biology processes or biochemical modifications are required. This reagent must be used with a magnetic plate.
Enzo Life Sciences Nupherin™ Transfection Reagent, (0.75mg)
Greatly enhances transfection efficiency. Use/Stability: On first use, aliquot the reagent and store at -80°C. Thawed aliquots of Nupherin™ can be frozen after use for. Long Term Storage or refrigerated for up to 1 week. Do not dilute reagent. Long Term Storage: -80°C
Lonza Walkersville SF Cell Line 4D-Nucleofector LV Kit XL, Catalog Number V4LC-2002, Reaction Volume Up to 20 mL, Kit Size 5 reactions, Processing Time Less than 10 min, Compatible System 4D-Nucleofector LV Unit
The Lonza SF Cell Line 4D-Nucleofector LV Kit XL is specifically designed for efficient, large-scale transfection of hard-to-transfect cell lines using the 4D-Nucleofector LV Unit. This closed-system nucleofection kit modifies up to 1 x 10^9 cells effectively, retaining cell viability. It allows variable reaction volumes and rapid processing, making it ideal for transient protein production and generating modified cell lines for various assays.
- Compatible system: 4D-Nucleofector LV Unit
- Reaction volume: Up to 20 mL
- Cell numbers supported: Up to 1 x 10^9 cells
- Processing time: Less than 10 minutes
- Kit size: 5 reactions
- Optimal cell lines: HEK293 suspension clones, CHO-S suspension clones, K-562, U-937
- Applications: Large scale transient protein production, generation of modified cell lines
- Key benefits: High transfection efficiencies, maintained cell functionality
- Storage conditions: 2 to 8 °C
Mayflower Bioscience LentiBOOST Research Grade Lentivirus Transduction Enhancer Solution, 200 Transductions
LentiBOOST is a chemical transduction enhancer simultaneously neutralizing electrostatic repulsion between cell membrane and virion and enhancing viral membrane fusion. In contrast to other chemical enhancers, LentiBOOST shows no toxic effects even in high concentrations. Therefore LentiBOOST is highly recommended for hard to transduce cells even in the presence of other transduction enhancers. LentiBOOST-R Research grade material is used for basic research only and is not transferable to the clinic. This is the latest technology which has been produced by Revvity and introduced by Mayflower Bioscience.
Lonza Walkersville SE Cell Line 4D-Nucleofector X Kit S
The SE Cell Line 4D-Nucleofector X Kit S is one of our three 4D-Nucleofector Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well Nucleocuvette Strip format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies in difficult-to-transfect cell linesConditions are transferable between 4D Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cellsMedium throughput applicationsOptimization of transfection conditions e.g. substrate titrationsContent:1 x 0.675 mL SE Cell Line Nucleofector Solution1 x 0.15 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0),2 x 16-well Nucleocuvette Strips (20 μL)
Andwin Scientific FectoPRO DNA Transfection Kit
The FectoPRO Transfection Kit is specifically designed for enhanced Transient Gene Expression (TGE) in suspension CHO and HEK-293 cells in various serum-free media, using low DNA amount.