Kit utilizes Live-dye™, a cell permeable green fluorescent dye (Ex(max): 488nm; Em(max): 518nm), to stain live cells. Dead cells can be easily stained by propidium iodide (PI), a red fluorescent dye (Ex(max): 488nm; Em(max): 615nm), which in viable cells is actively pumped out of the cytoplasm. Stained cells can be visualized by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine). Cyto-dye has also been used to discriminate between apoptotic and non-apoptotic cells. Ready-to-use. Applications: Fluorescence microscopy, Fluorescent detection .