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pHAb Amine Reactive Dye exhibits very low fluorescence at pH > 7 and much higher fluorescence as the pH of the solution becomes acidic. pHAb Dyes are designed specifically for antibody labeling.
The Viral ToxGlo™ Assay is a simple, quantifiable method of determining viral-induced cytopathic effects (CPE) in host cells caused by lytic virions. The assay measures cellular ATP as a surrogate measure of host cell viability.
Homogeneous, colorimetric cell viability assay; requires mixing of MTS and PMS components prior to assay, then a 1- to 4-hour incubation. Standalone MTS powder for applications where optimization of assay for specific cell types or conditions is desired.
A fast homogeneous, fluorometric method for estimating the number of non-viable cells present in multiwell plates. Based on detection of LDH release, an indicator of cytotoxicity.
The CytoTox-Fluor Cytotoxicity Assay is a single-reagent-addition, homogeneous, fluorescent assay that measures the relative number of dead cells in cell populations.
Colorimetric assay for the determination of cell membrane integrity based on detection of LDH release, an indicator of cytotoxicity. Sample transfer steps are required.
Luminescent ATP Cell Viability Assay Kit I uses thermostable firefly luciferase and purified luciferin substrate to quantitatively measure cellular ATP levels an indicator closely correlated with viable cell numbers and metabolic activity Upon reagent addition cells rapidly lyse to release intracellular ATP which interacts with luciferase and luciferin substrate generating a luminescent signal directly proportional to the number of viable cells The protocol does not require prior cell lysis steps offering a streamlined assay completed within 10 minutes This assay is suitable for detecting viability ranging from 10 to 30 000 cells and provides comparable results to common commercial ATP-based viability assays such as CellTiter-Glo
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ATP-Glo™ Bioluminometric Cell Viability Assay offers a highly sensitive assay for quantifying ATP. The homogeneous assay procedure involves a single addition of ATP-Glo™ Detection Cocktail directly to cells cultured in a serum-supplemented medium. It is not necessary to remove cell culture medium or wash cells before adding the reagent. This ATP detection kit takes advantage of firefly luciferase’s use of ATP to oxidize D-Luciferin and the resulting production of light in order to assess the amount of ATP available. The ATP-Glo™ kit can be used to detect as little as a single cell or 0.01 picomole of ATP. The signal produced is linear within 6 orders of magnitude. By relating the amount of ATP to the number of viable cells, the assay has wide applications, ranging from the determination of viable cell numbers to cell proliferation to cell cytotoxicity. This kit has reagents sufficient for 1000 assays in a 96-well format.
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Weve improved the Vi-CELL reagent pack to make it easier to use. The Vi-CELL reagents you know and trust are now packaged in a convenient ready-to-load kit. Simply open the instrument reagent panel and slide in the reagent pack. Each pack can be used for 310 activities before changing. Packs are easy to replace with no mess. Say goodbye to wrangling with tubes and splattering dye when you change out reagents. Your lab coat will thank you!Each reagent pack contains:Trypan Blue bottle.Waste bottle.Buffer solution bottle.Disinfectant bottle.Cleaning agent bottle.
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