Buffered Listeria Enrichment Broth (BLEB) Base is a selective enrichment broth used for the cultivation of Listeria species from food. Listeria are microaerophilic, gram-positive, asporogenous, non-encapsulated, non-branching, regular, short, motile rods. Motility is most pronounced at 20°C. The formulation is a modification of Listeria Enrichment Broth with added buffering strength. The addition of selective agents is delayed until the sample has been enriched for 4 hours in the broth. Sodium pyruvate is utilized to resuscitate stressed organisms. (The base does not include nalidixic acid, acriflavine hydrochloride, or cycloheximide.)• Product meets AOAC, APHA, BAM, EPA, NF, and SMWW reference methods and performance criteria.• For in vitro diagnostic (IVD) use.• BLEB Base should be used by trained professionals within a laboratory setting. • Product is not intended for use in the diagnosis or treatment of disease or other human conditions.