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Collections of defined cDNA fragments and open reading frame DNA sequences that have been cloned into vectors for the purpose of easy identification and isolation for further study. Ideal for use in cloning experiments.
Recombinant human FLT3 (Y591-V592insVDFREYEYD F691L) (571-993) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. This FLT3-ITD mutant has 9 amino acids “VDFREYEYD” repeated between the sites Y591 and V592 within the juxtamembrane domain.
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Recombinant human tag-free Tau-441 was co-expressed with DYRK1A in E. coli cells, phosphorylated by DYRK1A in vivo and in vitro, then the purified protein was biotinylated prior to the final chromatography purification.
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