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cDNA and ORF Libraries

Collections of defined cDNA fragments and open reading frame DNA sequences that have been cloned into vectors for the purpose of easy identification and isolation for further study. Ideal for use in cloning experiments.
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1

Invitrogen™ cDNA Size Fractionation Columns

Offers efficient and convenient method for size-fractioning double-stranded cDNA (1)

EDGE ENCOMPASS_PREFERRED
2

Invitrogen™ FirstChoice™ RLM-RACE Kit

Designed to amplify cDNA only from full-length, capped mRNA, usually producing a single band after PCR

EDGE ENCOMPASS_PREFERRED
Content And Storage

•FastAP™ Thermosensitive Alkaline Phosphatase
•10X FastAP Buffer, Tobacco Acid Pyrophosphatase
•10X TAP Buffer, T4 RNA Ligase
•10X T4 RNA Ligase Buffer
•M-MLV Reverse Transcriptase
•RNase Inhibitor
•10X RT Buffer
•2.5 mM dNTP Mix
•Mouse Thymus RNA (1 mg/ml)
•Random Decamers
•5' RACE Adapter
•3' RACE Adapter
•5' RACE Outer Primer
•5' RACE Inner Primer
•3' RACE Outer Primer
•3' RACE Inner Primer
•5' RACE Outer Control Primer
•5' RACE Inner Control Primer
•5' PCR Control Primer
•3' RACE Control Primer
•Ammonium Acetate Stop Solution
•Components should be stored at -20°C. Nuclease-free Water may be stored at any temperature.
Format Kit
Reverse Transcriptase M-MLV
Final Product Type cDNA
Product Type RLM-RACE Kit
Optimal Reaction Temperature 42°C
For Use With (Application) cDNA Libraries & Library Construction
Product Line Ambion, FirstChoice
3

MilliporeSigma™ Novagen™ T7Select™, 1-1 Cloning Kit

Uses T7 capsid protein to display peptides or proteins on surface of phage.

4

MilliporeSigma™ Novagen™ T7Select™ 10-3 Cloning Kit

Uses T7 capsid protein to display peptides or proteins on surface of phage.

5

Invitrogen™ 3' RACE System for Rapid Amplification of cDNA Ends

Converts up to 1μg of total RNA preparation or 100ng of control RNA into cDNA in one reaction

ENCOMPASS_PREFERRED
6

Invitrogen™ CloneMiner™ II cDNA Library Construction Kit

CloneMiner™ II cDNA Library Construction Kit

ENCOMPASS_PREFERRED
8

Invitrogen™ 5' RACE System for Rapid Amplification of cDNA Ends, version 2.0

Suitable for rapid amplification of cDNA ends (RACE) between defined point in mRNA and the 5' end

ENCOMPASS_PREFERRED
9

Promega pF3K WG (BYDV) Flexi™ Vector

Designed for use with Wheat Germ extracts and contain sequences from the barley yellow dwarf virus (BYDV), an RNA plant virus, upstream and downstream of the protein coding region of interest.

ENCOMPASS
10

Promega pFN2K (GST) Flexi™ Vector

These vectors are used for inducible expression of N-terminal GST-tagged fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter.

ENCOMPASS
11

Promega pFC14K HaloTag™ CMV Flexi™ Vector

Vectors designed for expression of C-terminal-tagged HaloTag™ fusion proteins in mammalian cells. Once expressed, use the HaloTag™ fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag™ Ligands.

ENCOMPASS
12

Promega pF4A CMV Flexi™ Vector

Designed for high-level expression of proteins in mammalian cells from the CMV promoter without a selectable marker

ENCOMPASS
13

Promega pF5K CMV-neo Flexi™ Vector

The Flexi Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences.

ENCOMPASS
14

Promega pF9A CMV hRluc-neo Flexi™ Vector

The Flexi Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences.

ENCOMPASS
15

Promega HaloTag™ Cloning Starter System

HaloTag™ Cloning Starter Kit: designed to use Flexi Cloning System to clone protein coding sequences into mammalian HaloTag™ expression vectors; clone and transfer gene sequences into C- or N-terminus of HaloTag™ Flexi CMV Deletion Vectors.

ENCOMPASS