Custom Peptide Synthesis and Protein Expression
Cayman Chemical Cel-free ProteIn SynthesIs 1ea
Cell-free protein synthesis (CFPS) is an EAsy-to-use, rapid, and efficient system that is devoid of membrane-bound barriers but contains the native cellular transcriptional and translational machinery and exogenous resources (e.g., amino acids, nucleotides, and a secondary energy substrate) needed for synthesis of the desired proteins.{71173,71174,71775,71176,71177} The CFPS (E. coli) Kit can produce high levels and different molecular weights of recombinant proteins within several hours and can be used in high-throughput functional genomics and proteomics. In addition, it can provide proteins to drive biochemical and structural biology research. The synthesized protein can be detected by SDS-PAGE, Western blot, and fluorescence-detection size exclusion chromatography (FSEC) and can be isolated from the reaction mixture by affinity purification techniques for further structural and/or functional characterization.
AAPPTec Kaiser Test Kit
The Kaiser Test Kit consists of: 50 mL of ninhydrin solution, and 50 mL of phenol + KCN solution. May be stored at room temperature for 6 months of greater.
Cosmo Bio Usa Inc DsbC Set (formerly DS supplement)
DS supplement is newly developed for use in cell-free coupled transcription/translation systems PUREfrex 2.0 and PUREfrex 2.1 to promote proper disulfide bond formation. DS supplement will be phased out and replaced with DsbC Set. Formation of disulfide bonds is important for the stability and activity of many proteins, including cell surface receptors and secreted proteins. Disulfide bonds are formed by oxidation of pairs of cysteine sulfhydryl (SH-) groups. Therefore, an oxidizing environment is necessary for disulfide bond formation. Additionally, the correct pairing of cysteines is promoted by Disulfide bond isomerase (e.g. E. coli DsbC) which can catalyze disulfide bond exchange reactions. DS supplement comprises highly purified E. coli DsbC and oxidized Glutathione (CSSG).
Invivogen STABLE EXPRESSION HUMAN NLRP1
A549-ASC-NLRP1 cells, Stable expression of human NLRP1 (isoform 1)
Takara Bio Aureobasidin A, 1mg
Aureobasidin A (AbA) is a cyclic depsipeptide antibiotic, isolated from the filamentous fungus Aureobasidium pullulans R106, which is toxic to yeast at low concentrations (0.1–0.5 μg/ml). Sensitive species include Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida glabrata, Aspergillus nidulans and A. niger. AbA inhibits a yeast enzyme, inositol phosphorylceramide (IPC) synthase, whichis expressed from the AUR1 gene. Expression of a mutant gene, AUR1-C, in transformed yeast confers resistance to the drug. 1mg
Sino Biological Cell-Free Protein Synthesis kit 50 Tests
CFKIT02 is a cell-free protein synthesis system based on E. coli lysate, supplemented with all the enzymes, energy regeneration system, and buffer environment required for efficient in vitro transcription and translation. Users only need to provide plasmid or PCR product templates to obtain soluble proteins within hours.
CEM Corporation Rink Amide ProTide Resin (LL), 25 g
ProTide resins contain an ideal PEG and polystyrene core leading to an optimized SPPS environment. Unmatched performance for routine synthesis of long (> 30 amino acids) or difficult peptide amides. Can be used in place of PAL or other Rink Amide resins.
Apexbio Technology LLC HBTU, 50g. Cas: 94790-37-1 MFCD: MFCD27957357. Peptide coupling reagent
IC50: Not available.HBTU (2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexauorophosphate), a coupling reagent commonly used in solid phase peptide synthesis. Other sizes are available. Please inqury us for quote.
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