DNA Vectors
Vector Biolabs FLAG tagged CRISPR/Cas9 nuclease RGD-fiber modified Adenovirus (Ad(RGD)-mCherry-FLAG-hCas9)
The Type II prokaryotic CRISPR/Cas system is the new class of tools for targeted genome engineering. The cas9 nucleases derived from clustered regularly interspaced short palindromic repeats (CRISPR)-cas systems uses small RNAs as guides (gRNA) to cleave DNA in a sequence-specific manner. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms. For site-specific genome editing, the CRISPR/Cas9 system requires the Cas 9 nuclease and the gRNA. This RGD-fiber modified adenovirus expresses a codon-optimized Cas9 nuclease with N-terminal FLAG tag and a mCherry reporter. It can be used along with guided RNA for genome-editing in cells that regular Ad5 doesn't infect well, including human or mouse macrophages, ES cells, T cells, synoviocytes, certain fibroblast and islet grafts etc.
SignaGen Laboratories AAV-NULL (AAV SEROTYPE 9)
Unit Size: 1 x >30 µL (>1E+13 VG/mL).AAV9-CMV-Null is a pre-packaged rAAV in serotype 9 (with capsid from AAV serotype 9 and 2xITR from AAV serotype 2) which carries no transgene under CMV promoter. Used as a control and ready to use format.
Boca Scientific Inc Exontrap Cloning Vector pET01, Lyophilized DNA, 5μg
The Exontrap vector pET01 has an intrinsic splicing function that enables selective cloning of exon sequences from large genomic eukaryotic DNA fragments. This new route for the identification of eukaryotic genes does not involve an initial isolation of cellular mRNA, allowing genes that are not transcribed during certain life cycle stages to be identified. This greatly facilitates exon/intron mapping. The Exontrap function is based on a shuttle vector containing prokaryotic and eukaryotic genetic elements for replication in both bacteria and cell cultures. The vector contains a 5´ and 3´ exon separated by a 600 bp intron sequence that has a polylinker for cloning.
G & P Biosciences HUMAN PD-L1 LENTIVIRUS, PUROM
Human PD-L1 Lentivirus, Full-length Gene in Lentivector, Pre-packaged Lentiviral Particles puromycin
New England Biolabs, Inc. PhiX174 RF I DNA – 150 µg
This is the double-stranded, covalently closed, circular form of X174 RF I DNA (supercoiled). The molecular weight of X174 RF I is 3.50 x 10^6 daltons and it is 5,386 base pairs in length. The vast majority of the molecules are RF I, and the remainder are RF II, as determined by agarose gel electrophoresis.
Gentarget GFP-Histone2B (Neo) Fusion, Concentrated Lentivirus, 200ul/vial (1×108 IFU/ml), (provided in PBS with 60ug/ml Polybrene (10x).
Pre-made lentivirus express a fusion target of (GFP-human Histone 2B). The human Histone 2B (H2B) was fusioned at its N-terminal with GFP, expressed under a constitutive suCMV promoter, containing a Neomycin antibiotic marker under the RSV promoter.Fluorescent labeled H2B allowed high-resolution imaging of both mitotic chromosomes and interphase chromatin, and the latter revealed various chromatin condensation states in live cells. It can directly observe the Double Minute Chromosomes (DMs) in living cells without compromising nuclear and chromosomal structures.