DNA Extraction and Purification
Biochain Institute Inc Genomic DNA - Mouse Normal Tissue: Lung, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Apexbio Technology LLC ApexPrep DNA Plasmid Miniprep Kit 300 tests
The ApexPrep DNA Plasmid Miniprep Kit is designed for isolation of plasmid DNA through alkaline-mediated bacterial cell lysis Upon lysis plasmid DNA selectively binds to a silica-based spin column under high-salt conditions separating it from cellular debris and contaminants Subsequent washing removes proteins and organic impurities yielding purified plasmid DNA (up to 30 g) from bacterial culture volumes of 1 5 mL Extracted plasmid DNA is suitable for downstream molecular biology applications including restriction digestion PCR amplification sequencing cloning transfection and in vitro transcription or translation assays
Biochain Institute Inc Genomic DNA - Parkinson's Disease: Brain: Thalamus, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
New England Biolabs, Inc. Monarch® 2 ml Tubes - 100 tubes
The Monarch 2 ml Tubes are supplied as a component of the Monarch HMW DNA Extraction Kits. The tubes are round-bottom and have a locking mechanism for safe and convenient handling. The tubes are low DNA binding, making them optimal for purification of HMW DNA in the Monarch workflows.
- A component of the Monarch HMW DNA Extraction Kits
- Low DNA binding
- Includes locking mechanism on the cap
- 2 ml capacity
Biotium AccuGreen™ Broad Range dsDNA Quantitation Kit (500 assays)
The AccuGreen™ Broad Range dsDNA Quantitation Kit is designed for use with handheld fluorometers such as the Qubit fluorometer from Thermo Fisher. The AccuGreen™ kit is specific for dsDNA, and can quantify DNA samples in the range of 0.2-100 ng/uL. The assay has a green fluorescence with an excitation/emission of 500/530 nm (with DNA). This kit has sufficient reagent for 500 assays.
Zymo Research Corporation ZymoPURE P1 (Red), 13 ml
ZymoPURE P1 (Red) is designed to be used with our ZymoPURE Plasmid Purification Kits, which is the fastest and simplest method available to efficiently isolate transfection-grade plasmid DNA from E. coli.
Norgen Biotek Corp Oligo Clean-Up/Concentrat Kit
This kit provides a rapid spin column procedure for the purification and concentration of up to 10 µg of oligonucleotides of various sizes. The kit is used for the purification of synthesized oligos, as well as the clean-up of oligos from various upstream enzymatic reactions such as ligation, Poly(A) tailing and end-labeling. Single or double stranded DNA or RNA oligos larger than 10 bp can be purified with this kit (not recommended for the removal of PCR primers). Oligos of up to 1000 bp have been tested with this kit.This kit purifies oligos from other reaction components including proteins, buffers and nucleotides without the use of phenol, chloroform, alcohol precipitation or urea PAGE extraction. The kit provides a high quality product with up to 90% recovery. Purified oligos can be used in a number of downstream applications such as end-point or quantitative reverse transcription PCR and RNAi studies.
New England Biolabs, Inc. NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® – 24 reactions
The Ultra II Directional RNA Library Prep Kit for Illumina delivers significantly increased sensitivity and specificity from your RNA-seq experiments, from ever-decreasing amounts of input RNA. In conjunction with ribosomal RNA (rRNA) depletion or poly(A) enrichment, the kit enables the production of high quality libraries from 5 ng or 10 ng of Total RNA, respectively, up to 1 µg. The NEBNext Ultra II Directional RNA Library Prep Kit derives its directionality from the “dUTP” method for strand-specificity, with proven superiority for this application.
- Generate the highest yields of high quality libraries, with a broad range of input amounts
- Increase the complexity and transcript coverage of your libraries
- Optimize your time with streamlined workflows, reduced hands-on time, and automation compatibility
- Rely on robust performance, even with low quality RNA, including FFPE
Zymo Research Corporation Human WGA Non-Methylated DNA (5 μg / 20 μl)
The Human WGA Methylated & Non-methylated DNA Set isgenerated using phi29 DNA polymerase based whole genome amplification techniques from HCT116 DKO cell line derived genomic DNA (Human HCT116 DKO Non-methylated DNA). The Human WGA Methylated DNA is Human WGA Non-methylated DNA that has been enzymatically methylated at all double-stranded CGdinucleotides using M.SssI methyltransferase and can be used as a positive control for DNA methylation analysis.
New England Biolabs, Inc. WarmStart LAMP Kit (DNA & RNA) – 500 reactions
The WarmStart LAMP Kit (DNA & RNA) is designed to provide a simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets. LAMP and RT-LAMP are commonly used isothermal amplification techniques that provides rapid detection of a target nucleic acid using LAMP-specific primers (supplied by the user) and a strand-displacing DNA polymerase. This kit is supplied with the WarmStart LAMP 2X Master Mix, which contains a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized LAMP buffer solution. Both Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase have been engineered for improved performance in LAMP and RT-LAMP reactions. A fluorescent dye is also supplied to enable real-time fluorescence measurement of LAMP. The WarmStart LAMP Kit is compatible with multiple detection methods, including turbidity detection, real-time fluorescence detection, and end-point visualization.
Agilent Technologies HS Large Fragment 50KB Kit, 500 samples, DNA Sizing Range: 75 bp - 50000 bp, DNA Sizing Accuracy: ± 15%, DNA Concentration Range: 5 pg/μL - 600 pg/μL, DNA Smear Range: 50 pg/μL - 5 ng/μL, Sample Volume Required: 2 μL
HS Large Fragment 50KB Kit, 500 samples, DNA Sizing Range: 75 bp - 50000 bp, DNA Sizing Accuracy: ± 15%, DNA Concentration Range: 5 pg/μL - 600 pg/μL, DNA Smear Range: 50 pg/μL - 5 ng/μL, Sample Volume Required: 2 μL
Biotium AccuGreen™ High Sensitivity dsDNA Quantitation Solution (500 assays)
The AccuGreen™ High Sensitivity dsDNA Quantitation solution is designed for use with handheld fluorometers such as the Qubit fluorometers from Thermo Fisher. Unlike absorbance-based measurements, AccuGreen™ Dye is highly selective for double-stranded DNA over single-stranded DNA or RNA. The AccuGreen™ High Sensitivity dsDNA Quantitation Solution includes AccuGreen™ dye and assay buffer, but no DNA standards. It is intended for users who wish to use their own DNA standard. The assay has a green fluorescence with an excitation/emission of 502/523 nm (with DNA). The assay has a linear range of 0.1-100 ng dsDNA and a sample concentration range between 5 pg/uL and 100 ng/uL. This product has sufficient reagent for 500 assays.
Biochain Institute Inc Genomic DNA - Liver Cirrhosis: Colon, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Alzheimer's Disease: Brain, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Multiple Sclerosis Disease: Brain: Frontal Lobe, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.