DNA Extraction and Purification
Biochain Institute Inc Genomic DNA - Arrhythmia, infarct: Heart Ventricle, left, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Mouse Normal Tissue: Stomach, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Zymo Research Corporation Pinpoint™ Slide DNA Isolation System (50 Preps) w/ Zymo-Spin™ I Columns (Uncapped)
The system combines our powerful Pinpoint tissuesampling technology with a one-step DNA extraction method and makes targeted tissue genomic DNA isolation easy and simple. The kit efficiently recovers genomic DNA from paraffin archives or fresh tissue sections for PCR analysis. Unlike the UV-fractionation method, the Pinpoint system does not destroy the surroundings of target cells. There is no need for expensive specialized equipment or computer software. Highlights- Easily isolates genomic DNA in any targeted microscopic tissue area on a slide.- The simple procedure combines Pinpoint tissue sampling technology with a one-step DNA extraction method.- Efficiently recovers genomic DNA from paraffin archives or fresh tissue sections for PCR analysis.
Magbio Genomics Inc HighPrep Blood & Tissue DNA Kit (96 preps)
The HighPrep™ Blood & Tissue DNA kit is a magnetic beads based system to process high quality genomic DNA from 50-250 µL of fresh or frozen whole blood, buffy coat (containing anticoagulants such as Citrate, EDTA and Heparin), lysate of tissues, mouse tails, cultured cells, saliva, or buccal swabs.The HighPrep Blood & Tissue DNA kit is highly geared for automation where high quality genomic DNA is reproducible in a high throughput environment. The kit is adaptable to commercially available liquid handling workstations.
Biotium Caldesmon, HMW (h-Caldesmon)(CALD1/820 + h-CALD), CF740 conjugate, 0.1mg/mL
Caldesmon HMW is the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low
New England Biolabs, Inc. NEBNext Ultra II DNA PCR-free Library Prep Kit for Illumina - 96 rxn
The NEBNext Ultra II DNA PCR-free Library Prep Kit contains the enzymes and buffers required to convert a broad range of pre-fragmented DNA input amounts into libraries for next-generation sequencing on the Illumina platform all without an amplification step. The fast user-friendly workflow requires minimal hands-on time and maintains excellent library yields and GC coverage without requiring PCR. Dont be biased The NEBNext Ultra II DNA PCR-free Library Prep Kit for Illumina can save you time and minimize amplification-induced sequence bias and errors with a complete solution for PCR-free NGS library preparation. Built on the robust and reliable Ultra II reagents and workflow from New England Biolabs you can trust that your samples are in the best hands your own.
Qiagen, Inc. ENDOFREE PLASMID MEGA KIT (5)
EndoFree Plasmid Mega Kit (5), Contains: 5 QIAGEN-tip 2500, 5 QIAfilter Mega-Giga Cartridges, reagents, certified endotoxin-free buffers, and a comprehensive handbook. For purification of low-copy plasmids and cosmids
Biochain Institute Inc Genomic DNA - Human Diabetic Diseased Tissue: Subcutaneous Adipose, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc FFPE and Frozen Matched Pair Genomic DNA: Human Adult Normal Tissue: Liver, 2 x 2 ug/pk
Matched Pair products include: Primary Pair (PP), or Primary and metastatic Pair (PM). PP consists of a pair of genomic DNA isolated from primary tumor and its adjacent normal tissue; PM consists of a pair of genomic DNA from primary tumor and corresponding metastatic tumor. Genomic DNA in each pair is prepared from the same donor. This product line is designed for identifying tumor-specific genes and tumor metastatic.
Biochain Institute Inc Express Cloning Checker Kit II: Green Solution, 1 ml/PK
BioChain's Express Cloning Checker Kits provide highly efficient methods of identifying recombinant colonies after transformation eliminating time-consuming plasmid preparation. In the large-scale screen method, the partial colonies of E. coli bacteria can be directly transfered from the transformation plates into the solutions of the kits and immediately run on an agarose gel electrophoresis. The results will be known within 1-2 hours instead of the 2 days required in a conventional method of labor-intensive plasmid DNA miniprep or colony hybridization.
Biochain Institute Inc Genomic DNA - Depression: Brain: Thalamus, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Zymo Research Corporation EZ-96 DNA Methylation MagPrep (4x96 rxns.)
The EZ-96 DNA Methylation MagPrep features a high-throughput (96-well), simplified procedure that streamlines bisulfite conversion of DNA. The kit is based on the three-step reaction that takes place between cytosine and sodium bisulfite during which cytosine is converted into uracil. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.