DNA Extraction and Purification
Biochain Institute Inc Genomic DNA - Liver Cirrhosis: Heart, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Lupus: Liver, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Research Products International Corp Oligo Clean & Concentrator, 200 Preps
The Oligo Clean & Concentrator provides a streamlined method for efficient recovery and clean-up of DNA/RNA fragments and oligonucletides ≥ 16 nt from labeling (radioactive, biotin, DIG, etc.) and other enzymatic reactions. Unincorporated nucleotides, short oligos, dyes, enzymes, and salts are effectively removed by the clean-up procedure. DNA/RNA is washed and concentrated into a small volume of water. Purified DNA/RNA, available in just 2 minutes, is suitable for hybridization, gel shift assays, enzymatic reactions, ligation, sequencing, microarray analysis, etc.
Fast & Simple: Clean and concentrate DNA and RNA oligonucleotides in 2 minutes.
Ultra-Pure: Ready for hybridization, Next-Gen Sequencing, PCR, ligations, etc.
Complete removal: Eliminate dyes, salts, enzymes, nucleotides, and short oligos.
Zymo Research Corporation ZymoBIOMICS DNA/RNA Mini Kit (50 preps)
The ZymoBIOMICS™ innovative lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. gram negative/positive bacteria, fungus, protozoans, and algae). The provided DNA/RNA Shield™ preserves nucleic acids at ambient temperatures, providing an unbiased molecular snapshot of the sample.The procedure uses Zymo-Spin™ column technology that results in highquality total RNA (including small/microRNAs 17-200 nt) that is free of PCR inhibitors (e.g. polyphenols, humic acids and fulvic acids) and is ready for RT-PCR, arrays, sequencing, etc
Bulldog Bio Inc FastGene Scriptase II 20,000 units at 200 U/µl (100 rxns)
How would you construct the ideal reverse transcriptase? It would have to make very long cDNAs at high yields. It would have to be able to work from very small amounts of input RNA. And of course, it would have to work fast. FastGene Scriptase II checks all three boxes, and it does it at a reasonable price. Here’s how. First, mutations are inserted into the RNase H domain of native MuLV‘s reverse transcriptase delivering a higher yield of full-length cDNAs. Next, changes are made to increase the enzymes natural thermal stability providing a more robust enzyme that doesn’t lose processing power, even at elevated temperatures. Finally, the reaction buffer composition is optimized for high speed production of cDNAs from incredibly low levels of input RNA. This unique combination of size, speed, and production makes FastGene Scriptase II the optimal choice for even the most complex applications requiring cDNAs, such as RT-qPCR and NGS.
Cayman Chemical Fungal Lysergol 100mg
An ergot alkaloid and 5-HT1A, 5-HT2A, and 5-HT2C receptor agonist (EC50s = 73, 1.6, and 6.6 nM, respectively, in a FLIPR assay); induces contractions in isolated rat tail arteries (EC50 = 436.5 nM); enhances the antibacterial activity of nalidixic acid against susceptible and drug-resistant E. coli at 10 UG/ml