DNA Extraction and Purification
Biochain Institute Inc Genomic DNA - Multiple Sclerosis Disease: Brain: Thalamus, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biotium RNase-Free Calf Thymus DNA, 1 mg/mL (1 mL)
During isolation of DNA, RNase treatment is performed to eliminate contaminating RNA. Because RNase activity is robust and resistant to denaturation, commercial preparations of DNA contain high levels of residual RNase activity, even after re-purification. Preparing DNA solutions in DEPC-treated water will not inactivate RNase already present in the DNA. Similarly, commercial RNase inhibitor additives are usually poorly effective, expensive, and interfere in downstream applications. Biotium has developed a unique proprietary method that irreversibly inactivates RNases in purified DNA. The resulting DNA solution is free of excess protein or other chemicals that may interfere with downstream applications. Our RNase-free Calf Thymus DNA solution undergoes stringent quality control testing to confirm the product is 100% free of RNase activity. The solution is provided as 1 mg/mL calf thymus DNA in TE. Store the product at 4⁰C.
Biochain Institute Inc Genomic DNA - Parkinson's Disease: Brain: Parietal Lobe, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Norgen Biotek Corp CleanAll DNA/RNA Clean-Up and Concentration Micro Kit
Norgen's CleanAll DNA/RNA Clean-Up and Concentration Micro Kit provides a rapid method for the purification, cleanup and concentration of RNA or DNA from different isolation methods or upstream applications. The kit can be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions. The kit purifies RNA from phenol/guanidine-based protocols or from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. Endotoxins can be removed from previously purified RNA solutions down to 0.1 EU/µg DNA or less. The kit can be used to clean up DNA from digestions, ligations, PCR reactions, DNA modification reactions and staining.Purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA and small interfering RNA.Purify all sizes of DNA, from small PCR products, native or linearized plasmids, oligonucleotides, to genomic DNA.
Research Products International Corp Zymoclean Gel DNA Recovery Kit w/ Zymo-Spin I Columns, Uncapped, 200 Preps
The Zymoclean Gel DNA Recovery Kit is a gel DNA extraction kit that provides rapid purification of high quality DNA from TAE/TBE-buffered agarose gels. This gel DNA extraction kit features Zymo-Spin technology to yield high-quality, purified DNA in just minutes. DNA purified using the Zymoclean Gel DNA Recovery kits is perfectly suited for use in DNA ligation reactions, sequencing, DNA labeling reactions, PCR, etc.
Quick recovery of ultra-pure DNA from agarose gels.
Column design permits DNA elution at high concentrations into minimal volumes.
Eluted DNA is well-suited for use in DNA ligation, sequencing, labeling, PCR, etc.
Lonza Walkersville TheraPEAK ACK Lysing Buffer 1x
Ammonium-Chloride-Potassium (ACK) Lysing Buffer is used to lyse red blood cells in preparations containing white blood cells Examples include bone marrow buffy coats whole blood treated with EDTA All TheraPEAK Products are produced according to applicable GMP standards and follow the USP/EP guidance for cell and gene therapy raw materials It is the end user s responsibility to ensure full compliance with local regulations in terms of safety assessments and effective removal prior to patient exposure TheraPEAK Media Products are produced according to applicable GMP raw material standards and are intended to support GMP manufacturing TheraPEAK Media Products are produced at FDA registered manufacturing sites with an ISO 13485 certified quality management system
Alkali Scientific Radiant™ Extract & Amplify Tissue PCR Kit 80 Reactions 2 x 1mL 2X HS Taq Red, 1 x 1.6mL 5X Extract Buffer A, 1 x .08mL 10X Extract Buffer B
Radiant™ Extract and Amplify Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tail, ear punch and other mammalian tissues, buccal swabs, hair shafts, and saliva. SPEED UP your GENOTYPING NO OVERNIGHT lysing Protocol: Extraction Solutions, included: 15 minutes then PCR as directed with supplied Radiant™ RED 2X mix During the quick 5 minute extraction process, DNA is released from the starting material by mixing the sample with ASI's proprietary Extraction Solution. This is followed by a 10 minute incubation at 95 degree to help inactivate proteins and genomic DNA. NO OVERNIGHT EXTRACTION. There is no need for mechanical disruption, organic extraction, column purification, or precipitation of the DNA.
Miltenyi Biotec, Inc. Multi-96 Columns, molecular (4x96)
Columns for automated isolation of epitope-tagged proteins, (co-)immunoprecipitation, chromatin immunoprecipitation, isolation of biotinylated molecules, mRNA, or HIV-1 with MultiMACS M96 Separators
Biochain Institute Inc Control Genomic DNA - Horse Male, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Control Genomic DNA - Hamster Male, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biotium Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) (rCALD1/820), CF740 conjugate, 0.1mg/mL
Caldesmon HMW is the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb This antibody recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detec
Biochain Institute Inc CancerSeq Plus Paraffin Tissue Tumor Section: Lung, 5 Curls/pk
To generate our CancerSeq line of tissues, genomic DNA was extracted from the FFPE tissues and validated by targeted NGS with Illumina's TruSeq Amplicon - Cancer Panel (TSACP), which includes 48 cancer-related genes. Our prescreened tissues come with detailed information regarding donor and cancer type, as well as NGS information such as chromosomal position, variant type (SNP or indel), alternate allele variations, quality score, depth of coverage, allele type, transcript ID and more. We offer the prescreened tissue as mounted sections or whole block. Please inquire for other options, such as curls or extracted nucleic acids.
Qiagen, Inc. DNEASY PLANT MAXI KIT (24)
24 DNeasy Maxi Spin Columns, 24 QIAshredder Maxi Spin Columns, RNase A, Buffers, Collection Tubes (50 ml).
Biochain Institute Inc Urine DNA Isolation Kit, 1 kit/PK
BioChain's Urine DNA Isolation Kit is designed for the purification of genomic DNA and circulating DNA from urine samples. Purification is performed with the use of Spin columns which bind the DNA. No phenol-chloroform, protease, or precipitation steps are involved in this procedure. Isolation of the DNA is performed by lysing the cells in a solution containing chaotropic salts and detergent. The DNA product can be used directly for quantitative PCR and other downstream applications.
Biochain Institute Inc Genomic DNA - Parkinson's Disease: Brain: Occipital Lobe, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.