DNA Extraction and Purification
Zymo Research Corporation ZymoBIOMICS 96 MagBead DNA Kit – Dx (Lysis Rack) (2 x 96 preps)
The ZymoBIOMICS DNA Kits are designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
Biochain Institute Inc Genomic DNA - Plant Normal Tissue: Wheat, 10 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Mouse Normal Tissue: Small Intestine, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Carnabio Usa Inc AURA(AURKA)/5UG/FULL-LENGTH
AurA(AURKA), Full-length, Wild type, Amino Acid 1-403, NP_940835.1, Expressed in Insect (sf21); 5microg
Bulldog Bio Inc FastGene Scriptase II ReadyMix (100 rxns), OdT & Random Hex-primers
How would you construct the ideal reverse transcriptase? It would have to make very long cDNAs at high yields. It would have to be able to work from very small amounts of input RNA. And of course, it would have to work fast. FastGene Scriptase II checks all three boxes, and it does it at a reasonable price. Here’s how. First, mutations are inserted into the RNase H domain of native MuLV‘s reverse transcriptase delivering a higher yield of full-length cDNAs. Next, changes are made to increase the enzymes natural thermal stability providing a more robust enzyme that doesn’t lose processing power, even at elevated temperatures. Finally, the reaction buffer composition is optimized for high speed production of cDNAs from incredibly low levels of input RNA. This unique combination of size, speed, and production makes FastGene Scriptase II the optimal choice for even the most complex applications requiring cDNAs, such as RT-qPCR and NGS.
Biochain Institute Inc Genomic DNA - Liver Cirrhosis: Liver, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Parkinson's Disease: Brain: Corpus Callosum, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc xTractPure FFPE RNA Extraction Kit, 1 kit/pk
xTractPure FFPE RNA Extraction Kit, 1 kit/pk
Bioworld Blood Genomic DNA Isolation Midi, 12 Prep(s)
For the rapid preparation of high quality DNA from whole blood, plasma and serum Input volumes of 500 µL to 2 mL No phenol required for DNA isolation Extract DNA from whole blood, plasma and serum Fast and convenient spin column protocol FEATURES AND BENEFITSNo Phenol-Chloroform extraction or alcohol precipitation - Isolate genomic DNA without the use of harmful chemicals Fast and easy processing - Rapid spin-column format allows for the processing of multiple samples in 30 minutes. Isolate genomic DNA from various inputs - Isolate DNA from various blood inputs including plasma, whole blood and serum Recovered genomic DNA is suitable for downstream applications - Purified genomic DNA is fully compatible with restriction enzyme digestions, Southern Blot and PCR analysis. High quality DNA with no RNA contamination - No contamination or degradation of genomic DNA isolated with the Blood Genomic DNA Isolation Midi Kit is observed.
Biochain Institute Inc Express Cloning Checker Kit I: Red Solution, 1 ml/PK
BioChain's Express Cloning Checker Kits provide highly efficient methods of identifying recombinant colonies after transformation eliminating time-consuming plasmid preparation. In the large-scale screen method, the partial colonies of E. coli bacteria can be directly transfered from the transformation plates into the solutions of the kits and immediately run on an agarose gel electrophoresis. The results will be known within 1-2 hours instead of the 2 days required in a conventional method of labor-intensive plasmid DNA miniprep or colony hybridization.