DNA Extraction and Purification
Research Products International Corp ZymoPURE II Plasmid Midiprep, 25 Preps
Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Midiprep kit provides the fastest and simplest method available to efficiently isolate up to 400 µg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the ZymoPURE II Midiprep kit can process up to 50 ml of culture in less than 20 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is bound onto a column with either a vacuum or a centrifuge instead of a lengthy gravity flow column. Additionally, there is no ethanol precipitation step required and the elution is performed using a microcentrifuge.
Teknova Diluent Solution. 1000mL, Sterile.
1.00% Bovine Serum Albumin(BSA), 0.05% (v/v) Tween-20, 135mM Sodium Chloride,2.7mM Potassium Chloride,4.3mM Disodium Phosphate,1.4mM Monopotassium Phosphate
Active Motif Nuclear Extract Kit
Active Motif's Nuclear Extract Kit isolates high-quality nuclear, cytoplasmic or whole-cell extract from mammalian cell or tissue samples. This eliminates the need for optimizing reagents and protocols, and ensures high yields every time. More importantly, standardizing your extraction procedure with our high-quality reagents ensures your reproducible results.
Integrated DNA Technologies Inc Alt-R™ HDR Enhancer V2, Increases HDR Efficiency, Lowers Cytotoxicity, 150 µL
The Alt-R™ HDR Enhancer V2 significantly boosts homology-directed repair (HDR) efficiency in genome editing workflows, providing improved cell viability with lower cytotoxicity compared to previous versions. Its versatility allows use with various delivery methods like electroporation and lipofection, making it ideal for multiple cultured human cell lines. This product uniquely combines effectiveness across diverse applications while minimizing off-target effects.
Zymo Research Corporation Femto™ Bacterial DNA Quantification Kit
Bacterial DNA can be reliably quantified in a background of non-bacterial DNA such as fungal, animal, and plant DNA, etc. This is essential for downstream applications that require accurate DNA input including quantifying bacteria DNA template for Next-generation sequencing library preparation and metagenomic analysis amongst others. With the Femto™ BacterialDNA Quantification Kit, one can dependably quantify as little as 20 fg DNA from 1 l purified from biological liquids, bacterial cultures, or environmental DNA samples.
Research Products International Corp EZ DNA Methylation-Gold Kit, 50 Rxns
The EZ DNA Methylation-Gold Kit is a refinement of our popular EZ DNA Methylation kit and uses heat denaturation instead of chemical denaturation of the input DNA. This allows for the denaturation and bisulfite conversion steps to be consolidated into one step, leading to a much-reduced incubation time. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.
Complete bisulfite conversion of DNA in less than 3 hours.
Desulphonation and recovery of bisulfite-treated DNA with a spin column.
Recovered DNA is ideal for downstream analyses such as PCR, endonuclease digestion, sequencing, microarrays, etc.
New England Biolabs, Inc. NEBNext Ultra™ II FS DNA Module – 24 reactions
This module is part of the NEBNext Ultra II FS workflow, and is optimized for use with the NEBNext Ultra II Ligation Module, for Illumina-compatible library construction.
Omega Biotek Inc Endo-Free Plasmid DNA Maxi Kit, 25 preps
Endo-Free Plasmid DNA Maxi Kit provides an efficient method for the large-scale isolation of low-endotoxin plasmid DNA. The system utilizes Lysate Clearance Filter Syringes instead of centrifugation to rapidly clear bacterial lysates post-alkaline lysis. Following lysate clearance, endotoxins are removed, and the protocol follows a simple “bind-wash-elute” procedure to deliver high-quality plasmid DNA.
New England Biolabs, Inc. Monarch® HMW DNA Extraction Kit for Tissue - 5 preps
The Monarch HMW DNA Extraction Kit for Tissue provides a rapid and reliable process for extracting high molecular weight (HMW), intact genomic DNA from various tissues and bacteria, as well as other sample types including yeast, insect, and amphibian. Optimized extraction protocol for tissue utilizes pestle homogenization and proteinase K digestion with agitation for sample lysis, followed by a protein removal step and precipitation of the extracted DNA onto the surface of large glass beads. DNA size ranges from 50 to greater than or equal to 500 kb for standard protocols and into the Mb range when the lowest agitation speeds are used for processing soft organ tissues and bacteria. Purified DNA is recovered in high yield with excellent purity, including nearly complete removal of RNA. Purified HMW DNA is suitable for a variety of downstream applications including long-read sequencing
Macherey-Nagel MN Square-well Block, 24 blocks
MN Square-well Block (24) 96-well blocks with 2.1 mL square wells pack of 24
Edge Biosystems Inc PERF. DTR ULTRA 96-WELL PLT KT
fully hydrated gel filtration matrix that provides for a convenient and simple method of dye terminator removal for sequencing cleanup.
