RNA Preparation and Purification
Biochain Institute Inc Total RNA - Liver Cirrhosis: Kidney, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Prehybridization Solution, 5 ml/PK
BioChain's IsHyb In Situ Hybridization Kit provides the reagents, including mRNA hybridization buffer, NBT/BCIP color development solution and washing buffer solutions, for prehybridization, hybridization, and post hybridization treatments.
New England Biolabs, Inc. NEBNext Multiplex Small RNA Library Prep Kit for Illumina Set 2 – 96 reactions
The novel NEBNext Small RNA workflow has been optimized to minimize adaptor-dimers while producing high-yield, high-diversity libraries. The NEBNext Small RNA Library Prep Set for Illumina (Set 1) includes adaptors and multiplex primers with 12 indices, to enable multiplexing.
New England Biolabs, Inc. NEBNext Ultra™ II RNA First Strand Synthesis Module – 24 reactions
This module is part of the Ultra II Directional RNA and Ultra II RNA workflows, for Illumina- compatible RNA library construction. These workflows are compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enable high yield preparation of high quality libraries from 5 ng - 1 g total RNA. The NEBNext Ultra II RNA First Strand Synthesis Module has been optimized to convert a broad range of input amounts of RNA into cDNA using random priming. The module is optimized for use with the NEBNext Ultra II Directional RNA Second Strand Synthesis Module or the NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module, and is part of the Ultra II RNA directional or non-directional workflows. These workflows are compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enable high yield preparation of high quality libraries from 5 ng - 1µg total RNA.
New England Biolabs, Inc. NEBNext Ultra™ II Directional RNA Second Strand Synthesis Module – 96 reactions
The NEBNext Ultra II Directional RNA Second Strand Synthesis Module has been optimized to generate double-stranded cDNA from first-strand cDNA, as part of the NEBNext Directional RNA library preparation workflow. This workflow uses the dUTP method for strand-specific library construction, which depends on incorporation of uracil into the second strand cDNA, enabled by the NEBNext Second Strand Synthesis Reaction Buffer with dUTP Mix, included in this module.
New England Biolabs, Inc. Monarch® Total RNA Miniprep Kit - 50 preps
The Monarch Total RNA Miniprep Kit is a comprehensive solution for sample preservation, cell lysis, gDNA removal, and purification of total RNA from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues. Additionally, tough-to-lyse samples, such as bacteria, yeast, and plant, can be processed with additional steps that enhance lysis. Total RNA, including viral RNA, can also be extracted from clinically relevant samples like saliva and swabs. Cleanup of enzymatic reactions or purification of RNA from TRIzol-extracted samples is also possible using this kit. Purified RNA is highly pure, has minimal residual gDNA and ranges in size from full-length rRNAs down to intact miRNAs. Additionally, differential binding conditions allow selective capture or exclusion of the sub-200 nucleotide RNA pool that includes miRNA, 5S rRNA, and tRNA. Purified RNA is suitable for downstream applications, such as RT-qPCR, RNA-seq, Northern blot analysis, etc.
Macherey-Nagel NucleoSpin RNA Plant, 50 preps
NucleoSpin RNA Plant (50) 50 preps for the isolation of RNA from plant - NucleoSpin RNA Plant Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase
Alkali Scientific RNA Extraction Spin Columns 2m
Alkali ScientificRNA Spin Columns provide a fast efficient and reliable method for extracting high-quality RNA from a variety of samples including tissues cells plants and blood Engineered with a robust polypropylene construction these columns ensure chemical resistance and sample integrity while their advanced silica membrane technology enables efficient RNA binding washing and elution Each unit is supplied with collection tubes for seamless processing in standard centrifuges These columns deliver highly pure RNA with reproducible yields suitable for downstream applications such as RT-PCR qPCR RNA sequencing and gene expression studies The DNase- and RNase-free construction ensures sample integrity while human DNA-free certification supports applications requiring high sensitivity and specificity Polypropylene Construction Durable and chemically compatible for reliable performance High Purity RNA Extracts intact RNA with minimal degradation ideal for advanced molecular applicati
New England Biolabs, Inc. NEBNext Cell Lysis Buffer Module – 96 reactions
This module contains the enzymes and buffers required to lyse isolated cultured and primary cells to extract RNA. The module is optimized for use with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina or NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module, and can be used to supplement the NEBNext Cell Lysis Buffer and Murine RNase Inhibitor components supplied in these products.
New England Biolabs, Inc. Monarch® Spin Columns S2A and Tubes - 100 preps
The Monarch Spin Columns S2A and Tubes are a component of Monarch kits for RNA cleanup, and also offered separately for convenience and flexibility. The columns are designed without the use of a retaining ring, helping to ensure no buffer retention and no carryover of contaminants.
- Monarch spin columns and collection tubes designed for nucleic acid purification
- Minimal to no buffer retention/risk of carry-over contamination
- Low elution volume from unique column design
- Included in Monarch kits, also offered separately for flexibility
- Made with less plastic than conventional columns from leading suppliers
Bioworld RNase AWAY™ 1L
RNase Away™ removes RNase and DNA contamination from glassware and plastic quickly and economically. Simply apply RNase away to surface or soak as needed and rinse with water. Non-abrasive formulation does not contain strong acids. Effective for use with electrophoresis equipment, glass plates, and other laboratory equipment. Specification: Removes RNase and DNA - Ready to use - Non-abrasive. Molecular Weight : 18.02 g/mol, pH : 6.0 - 8.0 at 25 °C, Solubility : completely miscible, Appearance Form : Liquid, Appearance Color : Clear, Melting Point : 0.0 °C, Boiling Point : 100.0 °C, Density : 1.023 g/cm3. 1L
Roche Diagnostics KAPA mRNA HyperPrep Kit, 24 libraries
KAPA RNA HyperPrep Kits include all reagents required for RNA fragmentation, cDNA synthesis, library preparation and amplification. KAPA Pure Beads are supplied in each kit for reaction cleanups, while KAPA Adapters are available separately. Kits also include upfront depletion modules for mRNA capture (KK8580, KK8581), rRNA depletion (KK8560, KK8561), and rRNA and globin depletion (KK8562, KK8563). *This item is non returnable.
GINKGO BIOWORKS INC (3P) 100 mL CFPS kit. 10,000 reactions of 10 uL. Contains lysate, reagent mix, and control plasmid.
Ginkgo’s cell free protein synthesis (CFPS) kit is an E. coli transcription/translation system optimized to produce proteins from a T7 RNA polymerase promoter. Reactions consist of a bacterial cell lysate and a reagent mix that contain all components required for protein production; the user must simply provide a DNA template that encodes the protein of interest under control of a T7 promoter. The system is easy-to-use, produces protein within a few hours, and is compatible with high-throughput liquid handling and automation platforms. Protein yield and function can be evaluated in solution or following purification.100 mL kit enables user to run 10,000 reactions of 10 µL, 2000 reactions of 50 µL, or user can scale reaction volumes to their own specifications. Storage at -80° C.
GINKGO BIOWORKS INC (3P) 10 mL CFPS kit. 1000 reactions of 10 uL. Contains lysate, reagent mix, and control plasmid.
Ginkgo’s cell free protein synthesis (CFPS) kit is an E. coli transcription/translation system optimized to produce proteins from a T7 RNA polymerase promoter. Reactions consist of a bacterial cell lysate and a reagent mix that contain all components required for protein production; the user must simply provide a DNA template that encodes the protein of interest under control of a T7 promoter. The system is easy-to-use, produces protein within a few hours, and is compatible with high-throughput liquid handling and automation platforms. Protein yield and function can be evaluated in solution or following purification.10 mL kit enables user to run 1000 reactions of 10 µL, 200 reactions of 50 µL, or user can scale reaction volumes to their own specifications. Storage at -80° C.
Biochain Institute Inc FFPE and Frozen Matched Pair Total RNA: Human Adult Normal Tissue: Liver, 2 x 1 ug/pk
The same normal tissue is collected with both FFPE and Snap Frozen format at the same time. Comparing the qRT-PCR or next generation sequencing data with total RNA isolated from both tissue format will help scientists to develope diagnostic method from FFPE samples, which are major clinical tissue sample