RNA Preparation and Purification
New England Biolabs, Inc. NEBNext® Ultra™ II Non-Directional RNA Second Strand Synthesis Module – 100 reactions
This module is part of the non-directional Ultra II RNA workflow, for Illumina- compatible non-strand-specific RNA library construction. This workflow is compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enables high yield preparation of high quality libraries from 5 ng – 1 µg total RNA.
- Designed for use in the NEBNext Ultra II non-directional RNA workflow
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Peripheral Blood Leukocyte, 10 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Brain: Parietal Lobe, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
GINKGO BIOWORKS INC (3P) 10 mL CFPS kit. 1000 reactions of 10 uL. Contains lysate, reagent mix, and control plasmid.
Ginkgo’s cell free protein synthesis (CFPS) kit is an E. coli transcription/translation system optimized to produce proteins from a T7 RNA polymerase promoter. Reactions consist of a bacterial cell lysate and a reagent mix that contain all components required for protein production; the user must simply provide a DNA template that encodes the protein of interest under control of a T7 promoter. The system is easy-to-use, produces protein within a few hours, and is compatible with high-throughput liquid handling and automation platforms. Protein yield and function can be evaluated in solution or following purification.10 mL kit enables user to run 1000 reactions of 10 µL, 200 reactions of 50 µL, or user can scale reaction volumes to their own specifications. Storage at -80° C.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue 5 Donor Pool: Placenta, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
New England Biolabs, Inc. NEBNext Cell Lysis Buffer Module – 96 reactions
This module contains the enzymes and buffers required to lyse isolated cultured and primary cells to extract RNA. The module is optimized for use with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina or NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module, and can be used to supplement the NEBNext Cell Lysis Buffer and Murine RNase Inhibitor components supplied in these products.
New England Biolabs, Inc. NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina – 96 reactions
The NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina uses a template switching method to generate full length cDNAs directly from single cells or 2 pg - 200 ng RNA, followed by conversion to sequence-ready libraries using the Ultra II FS workflow. This unique workflow enables generation of the highest yields from a broad range of inputs, and superior transcript detection, while providing reliably consistent performance. Please note that adaptors and primers are not included in the kit and are available separately.
MyBioSource RNA (For fixed/embedded tissues)(without Phenol & Chloroform, need not DNase I digestion, directly use in RT-PCR or qPCR) Isolation Kit, 50 Tests
This kit is designed to quickly extract total RNA from formalin-fixed and paraffin-embedded tissue samples. The unique lysis solution/proteinase K quickly lyses cells to release RNA, and then the lysis mixture passes through a genomic DNA removal column, where genomic DNA is removed and RNA is filtered through. After the filtered RNA is adjusted with ethanol to adjust the binding conditions, the RNA is selectively adsorbed on the silicon matrix membrane in the spin column in a high-isolated salt state, and then through a series of rapid rinsing-centrifugation steps, the protein-removing solution and the rinsing solution remove the cells. Metabolites, proteins and other impurities are removed, and finally the low-salt RNase free H20 will elute the pure RNA from the silicon matrix membrane. The obtained RNA can be used in experiments such as reverse transcription PCR and fluorescent quantitative PCR.
Biochain Institute Inc Total RNA - Plant Normal Tissue: Soy bean, 100 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue 5 Donor Pool: Small Intestine, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Zyagen Labs Rat Liver Genomic DNA, 0.1mg
Rat Liver genomic DNA is a highly pure intact high molecular size DNA. It is extracted from single tissue of single donor and treated with DNase-free RNase to remove contaminant RNA. Genomic DNA is precisely measured by Nanodrop (a spectrophotometer technology) and stored at -80oCApplications:PCR Amplification, Southern blot analysis, genomic DNA methylation, single-nucleotide polymorphism (SNP) Analysis, profiling studies in gene expression, and DNA library construction
Biochain Institute Inc Total RNA - Rat Normal Tissue: Small Intestine, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
New England Biolabs, Inc. NEBNext Ultra™ II RNA First Strand Synthesis Module – 24 reactions
This module is part of the Ultra II Directional RNA and Ultra II RNA workflows, for Illumina- compatible RNA library construction. These workflows are compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enable high yield preparation of high quality libraries from 5 ng - 1 g total RNA. The NEBNext Ultra II RNA First Strand Synthesis Module has been optimized to convert a broad range of input amounts of RNA into cDNA using random priming. The module is optimized for use with the NEBNext Ultra II Directional RNA Second Strand Synthesis Module or the NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module, and is part of the Ultra II RNA directional or non-directional workflows. These workflows are compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enable high yield preparation of high quality libraries from 5 ng - 1µg total RNA.
Biochain Institute Inc Total RNA - Mouse Normal Tissue: Lung, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.