RNA Preparation and Purification
Macherey-Nagel NucleoSpin RNA Plant, 50 preps
NucleoSpin RNA Plant (50) 50 preps for the isolation of RNA from plant - NucleoSpin RNA Plant Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase
Zyagen Labs Mouse C57 spleen total RNA is a high pure intact total RNA isolated from freshly harvested normal healthy C57BL/6 Mouse SPLEEN tissues, treated with DNase and dissolved in water. RNA is ready for immediate use in any downstream application.
Zyagen Total RNA is routinely extracted from freshly harvested normal healthy tissues of single donor using classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. Total RNA sample is provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.
New England Biolabs, Inc. Monarch® Buffer BX - 80 ml
The Monarch Buffer BX, a component of Monarch Nucleic Acid Purification Kits, is a guanidine-based buffer designed for binding steps in nucleic acid purification.
- A component of the Monarch RNA Cleanup Kits
- A guanidine-based buffer designed for binding steps in nucleic acid purification
- Monarch kit components are also available separately for added convenience
- Monarch kit components are also available separately for added convenience
MyBioSource RNA (For fixed/embedded tissues)(without Phenol & Chloroform, need not DNase I digestion, directly use in RT-PCR or qPCR) Isolation Kit, 50 Tests
This kit is designed to quickly extract total RNA from formalin-fixed and paraffin-embedded tissue samples. The unique lysis solution/proteinase K quickly lyses cells to release RNA, and then the lysis mixture passes through a genomic DNA removal column, where genomic DNA is removed and RNA is filtered through. After the filtered RNA is adjusted with ethanol to adjust the binding conditions, the RNA is selectively adsorbed on the silicon matrix membrane in the spin column in a high-isolated salt state, and then through a series of rapid rinsing-centrifugation steps, the protein-removing solution and the rinsing solution remove the cells. Metabolites, proteins and other impurities are removed, and finally the low-salt RNase free H20 will elute the pure RNA from the silicon matrix membrane. The obtained RNA can be used in experiments such as reverse transcription PCR and fluorescent quantitative PCR.
Zyagen Labs Mouse C57 heart total RNA is a high pure intact total RNA isolated from freshly harvested normal healthy C57BL/6 Mouse HEART tissues, treated with DNase and dissolved in water. RNA is ready for immediate use in any downstream application.
Zyagen Total RNA is routinely extracted from freshly harvested normal healthy tissues of single donor using classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. Total RNA sample is provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.
Zyagen Labs Mouse C57 spinal cord RNA is a high pure intact total RNA isolated from freshly harvested normal healthy C57BL/6 Mouse SPINAL CORD tissues, treated with DNase and dissolved in water. RNA is ready for immediate use in any downstream application.
Zyagen Total RNA is routinely extracted from freshly harvested normal healthy tissues of single donor using classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. Total RNA sample is provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.
Alkali Scientific RNA Extraction Spin Columns 2m
Alkali ScientificRNA Spin Columns provide a fast efficient and reliable method for extracting high-quality RNA from a variety of samples including tissues cells plants and blood Engineered with a robust polypropylene construction these columns ensure chemical resistance and sample integrity while their advanced silica membrane technology enables efficient RNA binding washing and elution Each unit is supplied with collection tubes for seamless processing in standard centrifuges These columns deliver highly pure RNA with reproducible yields suitable for downstream applications such as RT-PCR qPCR RNA sequencing and gene expression studies The DNase- and RNase-free construction ensures sample integrity while human DNA-free certification supports applications requiring high sensitivity and specificity Polypropylene Construction Durable and chemically compatible for reliable performance High Purity RNA Extracts intact RNA with minimal degradation ideal for advanced molecular applicati
Epoch Life Science Inc GenCatch™ Total RNA Extraction System (250 preps) Uniquely formulated buffers, reinforced columns with collection tubes, along with protocols.
Provides a simple, fast and cost-effective method to purify total RNA from various starting materials (such as cultured cells, tissues, bacteria, yeast, etc.). Total RNA with a molecular size greater than 200 nucleotides (which excludes smaller RNA such as 5S and 5.8S RNA, and tRNA) can be isolated. Silica membrane technology. Up to 65 ug yield. 200 ul elution volume. No phenol/chloroform extraction nor ethanol precipitation required. Instructions for sample type listed in protocol. Used by labs around the world. 18 month shelf life.
New England Biolabs, Inc. 3'-O-Me-m7G(5')ppp(5')G RNA Cap Structure Analog – 5 umol
Blocking of the 3' -hydroxyl of m7G with 3' -0-Me assures that the capped transcripts are homogeneous. The 3' - hydroxyl of the non-methylated G is the only 3' - hydroxyl available for initiation. The 5' terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level. For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation, and a decrease in the formation of the initiation complex of mRNAs for protein synthesis. Certain prokaryotic mRNAs containing a 5 terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system. Also a cap requirement has been observed for splicing eukaryotic substrate RNAs. A method using E. coli RNA Polymerase primed with m7G(5' )ppp(5' )G or m7G(5' )ppp(5' )A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas.
New England Biolabs, Inc. NEBNext rRNA Depletion Kit (Bacteria) – 6 reactions
The NEBNext rRNA Depletion Kit (Bacteria) employs the NEBNext RNase H-based RNA depletion workflow to target removal of rRNA from gram-positive and gram-negative organisms. The method is effective with intact and degraded RNA, from monocultures or samples with mixed bacterial species (e.g., metatranscriptomic).
New England Biolabs, Inc. NEBNext rRNA Depletion Kit v2 (Human, Mouse, Rat) with RNA Sample Purification Beads – 96 reactions
The NEBNext rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads employs an RNaseH-based method to deplete both cytoplasmic (5S rRNA, 5.8S rRNA, 18S rRNA and 28S rRNA) and mitochondrial ribosomal RNA (12S rRNA and 16S rRNA) from human, mouse and rat total RNA preparations. This product is suitable for both intact and degraded RNA (e.g., FFPE RNA). The resulting rRNA-depleted RNA is suitable for RNA-Seq, random-primed cDNA synthesis, or other downstream RNA analysis applications. For added convenience, this kit contains NEBNext RNA Sample Purification Beads (Agencourt RNAClean XP beads from Beckman Coulter).