RNA Preparation and Purification
Zymo Research Corporation Direct-zol-96 MagBead RNA (4x 96 preps)
The extraction method inactivates viruses and other infectious agents. Total RNA (including small/microRNAs) are effectively isolated from a variety of sample sources (cells, tissues, biological liquids, DNA/RNA Shield™ samples, etc.). The procedure is easy: simply add ethanol and MagBinding Beads to a sample in TRIzol/TRI Reagent, wash and elute the RNA. No chloroform, phase separation or precipitation steps are necessary. High-quality total RNA is ready for Next-Gen sequencing, RT-qPCR, transcription profiling, hybridization, etc
New England Biolabs, Inc. NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) – 96 reactions
The NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) employs the NEBNext RNase H-based RNA depletion workflow to deplete adult, embryonic and fetal globin mRNA (HBA1/2, HBB, HBD, HBM, HBG1/2, HBE1, HBQ1 and HBZ), cytoplasmic rRNA (5S, 5.8S, 18S, 28S, ITS and ETS) and mitochondrial rRNA (12S and 16S). The kit is effective with human, mouse and rat total RNA preparations, both intact and degraded. The resulting depleted RNA is suitable for RNA-seq, random-primed cDNA synthesis, or other downstream RNA analysis. This kit can also be used following poly(A) mRNA enrichment (e.g., using the NEBNext poly(A) mRNA Magnetic Isolation Module NEB #E7490), so that the final depleted RNA contains only mRNA of interest and no non-coding RNA.
Zymo Research Corporation ZR RNA Buffer, 50 ml
ZR RNA Buffer is a one-step lysis and binding buffer for the purification of RNA in the Quick-RNA Midiprep Kit.
New England Biolabs, Inc. NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina -24 rxns – 24 reactions
The NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina uses a template switching method to generate full length cDNAs directly from single cells or 2 pg - 200 ng RNA, followed by conversion to sequence-ready libraries using the Ultra II FS workflow. This unique workflow enables generation of the highest yields from a broad range of inputs, and superior transcript detection, while providing reliably consistent performance. Please note that adaptors and primers are not included in the kit and are available separately.
![Zymo Research Corporation Direct-zol-96 MagBead RNA (4x96 preps) w/ TRI Reagent [Includes R2050-1-200 x 2 – packaged separately]](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502037264.JPG-250.jpg)
Zymo Research Corporation Direct-zol-96 MagBead RNA (4x96 preps) w/ TRI Reagent [Includes R2050-1-200 x 2 – packaged separately]
The extraction method inactivates viruses and other infectious agents. Total RNA (including small/microRNAs) are effectively isolated from a variety of sample sources (cells, tissues, biological liquids, DNA/RNA Shield™ samples, etc.). The procedure is easy: simply add ethanol and MagBinding Beads to a sample in TRIzol/TRI Reagent, wash and elute the RNA. No chloroform, phase separation or precipitation steps are necessary. High-quality total RNA is ready for Next-Gen sequencing, RT-qPCR, transcription profiling, hybridization, etc
Research Products International Corp Direct-zol RNA MicroPrep w/Zymo-Spin IC Columns, Capped, 50 Preps
The Direct-zol RNA MicroPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 10 µg (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis.
Molecular Research Center TRI REAGENT BD 100ML
For single-step isolation of total RNA or simultaneous isolation of RNA, DNA and proteins from whole blood, plasma, or serum. 0.75 ml of TRI Reagent BD processes 0.25 ml of blood derivatives.
Qiagen, Inc. RNeasy Plus Mini Kit, for 50 minipreps
Kit, RNeasy Plus Mini; For purification of total RNA from cultured cells and tissues using gDNA Eliminator columns; 50 RNeasy Mini Spin Columns, 50g DNA Eliminator Mini Spin Columns, Collection Tubes, RNase-Free Reagents and Buffers, 1/KT
Molecular Research Center TRI REAGENT RT 50ML
A reagent for the single-step isolation of RNA from tissues and cells. 1 ml of TRI Reagent RT processes 50 mg tissue or 107 cells. The isolated RNA is free of DNA contamination and ready for RT-PCR without the DNase treatment.
Research Products International Corp RNA Clean & Concentrator-5 w/Zymo-Spin IC Columns, Capped, 200 Preps
The RNA Clean & Concentrator kits are RNA clean up kits that provide a simple and reliable method for the rapid preparation of high-quality RT-PCR-ready, DNA-free (R1013, R1014) RNA. This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt). The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA. The RNA can be eluted from the Zymo-Spin IC Column in as little as ≥ 6 µl of RNase-free water. The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.
NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
Ultra-Pure: Eliminate contaminants and inhibitors in 5 minutes.
Qiagen, Inc. RNEASY MIDI KIT (50)
RNeasy Midi Kit (50), for total RNA isolation from a wide variety of sources. Contains: 50 RNeasy Midi Spin Columns, RNase-free reagents, buffers, collection tubes and a comprehensive handbook.
Norgen Biotek Corp RNA Clean-Up and Concentration Kit, 50 Prep
This kit is designed to clean-up and concentrate RNA (including miRNA) from TRIzol and TRI Reagent, enzymatic reactions, in vitro transcription, labelling reactions, etc. This is a robust kit for all clean-up and concentration purposes for up to 35 µg of RNA in solution. The purified RNA is of the highest purity and integrity, and can be used in a number of downstream applications.The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to miRNA, siRNA and lncRNA.The kit elutes RNA in 20 µL for all types of downstream applications. For smaller elution volumes of 8 µL, please refer to the microspin column kits such as the RNA Clean-Up and Concentration Micro-Elute Kit for the highest concentration of RNA sample.