RNA Preparation and Purification
Takara Bio NUCLEOSPIN RNA FLTR PLTE PK/4
NucleoSpin RNA Filter Plates (4) 96-well plates for filtration of cell and tissue homogenates pack of 4
New England Biolabs, Inc. NEBNext RNA Depletion Core Reagent Set - 24 rxns
In RNA-seq, highly expressed transcripts with minimal biological interest, such as ribosomal RNA (rRNA) can dominate readouts and mask detection of more informative low-abundance transcripts. This challenge is amplified when working with sample types for which pre-designed RNA depletion kits are not available. The NEBNext RNA Depletion Core Reagent set provides a customized approach to deplete unwanted RNA from any organism, using probe sequences designed with the user-friendly NEBNext Custom RNA Depletion Design Tool. The efficient RNase-H-based workflow, and close tiling of probes designed using the online tool, enables effective depletion from both low- and high-quality RNA, with a broad range of input amounts. The kit is also available with RNAClean beads.
Zyagen Labs Mouse C57 thyroid total RNA is a high pure intact total RNA isolated from freshly harvested normal healthy C57BL/6 Mouse THYROID tissues, treated with DNase and dissolved in water. RNA is ready for immediate use in any downstream application.
Zyagen Total RNA is routinely extracted from freshly harvested normal healthy tissues of single donor using classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. Total RNA sample is provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.
Zyagen Labs Dog spleen total RNA is a high pure intact total RNA isolated from freshly harvested normal healthy beagle DOG SPLEEN tissues, treated with DNase and dissolved in water. RNA is ready for immediate use in any downstream application.
Zyagen Total RNA is routinely extracted from freshly harvested normal healthy tissues of single donor using classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. Total RNA sample is provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.
Zyagen Labs MiniPig cerebral cortex RNA is a high pure intact total RNA isolated from freshly harvested healthy Gottingen MINIPIG CEREBRAL CORTEX tissues, treated with DNase and dissolved in water. RNA is ready for immediate use in any downstream application.
Zyagen Total RNA is routinely extracted from freshly harvested normal healthy tissues of single donor using classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. Total RNA sample is provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.
Zyagen Labs Monkey Universal Reference Total RNA extracted from wide variety of male and female Cynomolgus MONKEY tissues. Made at industrial scales to minimize lot-to-lot variation. Ideal for microarray, NB, PCR and many other applications. 0.1mg/pK at 1mg/ml
Zyagen Universal Reference Total RNA is composed of highly pure intact total RNA isolated from wide variety of male & female tissues. Total RNA is extracted using modified guanidine thiocyanate method, treated with DNase & packed at a concentration of 1mg/ml. Features: High quality intact RNA without genomic DNA contamination. Consistent control for standard data set comparisons. Extracted from large variety of male & female tissues for broad gene coverage. Made at industrial scales to minimize lot-to-lot variation Quality Control: The integrity of RNA sample as indicated by intact ribosomal RNA is verified by agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. Applications: Reference RNA is ideal for microarray analysis, Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, microRNA studies, & purification of mRNA for library construction.
New England Biolabs, Inc. Monarch® Spin RNA Cleanup Kit (10 ug) - 100 preps
The Monarch Spin RNA Cleanup Kit (10 ug) rapidly and reliably purifies up to 10 ug of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. The kit can also be applied for total RNA extraction from some sample types. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design helps ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 ul. Eluted RNA is ready for use in a variety of downstream applications, including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (>= 15 nts). Monarch Spin RNA Cleanup kits are also available for 50 ug and 500 ug binding capacities. Columns and buffers are also available separately for convenience
Macherey-Nagel NucleoSpin RNA Clean-up, 250 preps
NucleoSpin RNA Clean-up (250) 250 preps for clean-up of total RNA from reaction mixtures - NucleoSpin RNA Columns, Collection Tubes, buffers
Macherey-Nagel NucleoSpin RNA Clean-up, 50 preps
NucleoSpin RNA Clean-up (50) 50 preps for clean-up of total RNA from reaction mixtures - NucleoSpin RNA Columns, Collection Tubes, buffers
System Biosciences LLC SeraMir Exosome RNA Purification Column Kit (20 exoRNA columns), 20 Preps
Efficiently purify RNA from already isolated exosomes with SBI’s most flexible SeraMir Kit.
Roche Diagnostics KAPA mRNA HyperPrep (96rxn) Kit
KAPA mRNA HyperPrep Kits include all reagents required for mRNA Capture, RNA fragmentation, cDNA synthesis, library preparation and amplification. KAPA Pure Beads are supplied in each kit for reaction cleanups while KAPA Adapters are available separately. KAPA mRNA HyperPrep (96rxn) Kit includes three component kits: mRNA Capture 96 rxn Kit, RNA Hyper 96 rxn Kit, and 3 x 5mL Pure Beads. *This item is non returnable.
Zyagen Labs Human skeletal muscle RNA is a high pure intact total RNA isolated from freshly harvested normal healthy HUMAN SKELETAL MUSCLES tissues, treated with DNase and dissolved in water. RNA is ready for immediate use in any downstream application.
Zyagen Total RNA is routinely extracted from freshly harvested normal healthy tissues of single donor using classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. Total RNA sample is provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.