RNA Preparation and Purification
Research Products International Corp Direct-zol RNA MiniPrep w/Zymo-Spin IIC Columns, Capped, 200 Preps
The Direct-zol RNA MiniPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary.
Zymo Research Corporation Direct-zol™ RNA MiniPrep (200 Preps) w/ Zymo-Spin™ IIC Columns (Capped)
Total RNA including small RNAs (17-200 nt)2, is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, samples stored in DNA/RNA Shield™, etc.).Simply add ethanol to a TRI Reagent sample, bind directly to the Zymo-Spin™ Column, wash, and elute RNA. No phase separation, precipitation, or post-purification steps are necessary. RNA is high-quality and ready for Next-Gen Sequencing, RTqPCR, transcription profiling, hybridization, etc.
Zymo Research Corporation Quick-RNA™ MicroPrep Kit (50 Preps) w/Zymo-Spin™ IC Columns (Capped)
The Quick-RNA Microprep Kit is designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 106) and tissue samples (up to 5 mg). The procedure combines a unique buffer system with Zymo-Spin™ column technology to yield high quality total RNA (including small RNAs 17-200 nt) in about 10 minutes. The procedure is simple. Add the provided RNA Lysis Buffer to a sample, and then purify the RNA using the Zymo-Spin™Columns. The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing etc. In addition, the kit can be used for the enrichment of small and large RNAs into separate fractions.
Zymo Research Corporation Quick-RNA Plant MiniPrep™ Kit (50 Preps)
The kit includes unique technology such as the ZR BashingBead™ Lysis Tubes and features a specially formulated RNA Lysis Buffer. The Zymo-Spin™ IIICG Column allows for high-capacity DNA elimination and the subsequent Zymo-Spin™ IICR Column efficiently adsorbs total RNA.The RNA is washed and then eluted with DNase/RNase-Free Water. For inhibitor removal, the eluted RNA can be treated by running the sample through the Zymo-Spin™ III-HRC Filters. RNA is eluted in as little as 6 l and is suitable for subsequent procedures including RT-qPCR.
Fisher BioReagents™ SurePrep™ RNA/Protein Purification Kit
Rapid method for the isolation and purification of total RNA and proteins sequentially from a single sample using the same column in less than 20 minutes
Fisher BioReagents™ SurePrep™ Nuclear or Cytoplasmic RNA Purification Kit
Rapid method for the isolation and purification of both nuclear and cytoplasmic RNA from cultured animal cells and animal tissue samples
Fisher BioReagents™ SurePrep™ RNA Cleanup and Concentration Kit
Provides a rapid method to purify and concentrate RNA isolated using other methods such as phenol:chloroform extraction or after enzymatic reactions
MP Biomedicals™ FastRNA™ Win Kit
For easy and quick isolation of total RNA from a wide variety of plant samples
Fisher BioReagents™ SurePrep™ RNA/DNA/Protein Purification Kit
Rapid method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, tissue samples, blood, bacteria, yeast, fungi or plants
MilliporeSigma™ Novagen™ T7 Tail Fiber Monoclonal Antibody
Used to immunoprecipitate T7 phage or T7 tail-fiber from solution.
New England Biolabs, Inc. NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads - 24 rxns
In RNA-seq, highly expressed transcripts with minimal biological interest, such as ribosomal RNA (rRNA) can dominate readouts and mask detection of more informative low-abundance transcripts. This challenge is amplified when working with sample types for which pre-designed RNA depletion kits are not available. The NEBNext RNA Depletion Core Reagent set provides a customized approach to deplete unwanted RNA from any organism, using probe sequences designed with the user-friendly NEBNext Custom RNA Depletion Design Tool. The efficient RNase-H-based workflow, and close tiling of probes designed using the online tool, enables effective depletion from both low- and high-quality RNA, with a broad range of input amounts. The kit is also available with RNAClean beads.