Miscellaneous Molecular Biology Reagents
BD Pharmingen™ Hoechst 34580 Reagent
For the fluorescent staining of DNA and nuclei in live or fixed cells. Hoechst 34580 is a bisbenzimidazole dye with high specificity for binding to double-stranded DNA.
MilliporeSigma™ NovaBiochem™ Deblock Reagent, 10% DCA in Toluene
Used for synthesis of DNA
Thermo Scientific Chemicals Ethidium Bromide, Electrophoresis Reagent, 98% (dry weight)
CAS: 1239-45-8 Molecular Formula: C21H20BrN3,C21H20BrN3 Synonym: 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide; Homidium bromide
MilliporeSigma™ Calbiochem™ SPUTOLYSIN™ Reagent
For isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum
MilliporeSigma™ Oxidizing Reagent(0.5M), I2 in Pyridine
Used for synthesis of DNA
Thermo Scientific Chemicals Triton∣r X-100, Electrophoresis Reagent
CAS: 9002-93-1 Molecular Formula: C16H26O2,C16H26O2 Synonym: Octoxynol-9; Polyethylene glycol tert-octylphenyl ether
MilliporeSigma™ Novabiochem™ Oxidizing Reagent, 0.05M, I2 in Pyridine
Used in biosynthesis.
MilliporeSigma™ Calbiochem™ FICOLL™ 400 Reagent, Molecular Biology Grade
Separation of lymphocyte subpopulations
Promega Integrated Reagent Caps for ReliaPrep™ Large Volume HT gDNA Isolation System
Accessory for use with the ReliaPrep Large Volume HT gDNA System used in automated purification of DNA from 1-10 mL blood on liquid-handling systems.
Thermo Scientific™ DTT (dithiothreitol)
Stabilize enzymes and other proteins which possess free sulfhydryl groups with DTT, shown to restore activity lost by oxidation of these groups in vitro.
Invitrogen™ UltraPure™ BSA (50 mg/mL)
Ambion Ultrapure BSA is a 'non-acetylated' BSA pure enough to use when DNA or RNA integrity is critical.
Invitrogen™ NaCl (5 M), RNase-free
Invitrogen Molecular biology grade, 5 M NaCl solution is supplied in one bottle. The solution is certified RNase-free, economical, and ready-to-use.
Thermo Scientific™ MgCl2 (magnesium chloride) (25 mM)
Thermo Scientific 25 mM MgCl2 is an aqueous solution of 0.22 μm membrane-filtered magnesium chloride, used for optimization of magnesium ion concentration in PCR.
Invitrogen™ MgCl2 (1 M)
Molecular biology grade, Invitrogen 1M MgCl2 solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use.