Lyo-Ready dUTP 1-Step RT-qPCR Mix combines the latest advances in buffer chemistry and PCR enhancers, together with hot start polymerase, optimized dNTP/dUTP mix, and separate lyo-compatible reverse transcriptase and dilution buffer. Lyo-Ready dUTP 1-Step RT-qPCR Mix is designed for stringent amplification of low copy-number RNA and DNA targets, it is ideal for high-throughput, automated platforms. It can be used as a wet mix or it can be lyophilized without impacting its performance post rehydration.