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Nucleotides are composed of a nitrogenous base covalently bound to a 5-carbon sugar molecule and one or more phosphate functional groups. They are the molecules that make up the structural units of DNA and RNA.
ARCA EGFP mRNA is a reporter mRNA suitable as a control reagent for studying transfection and expression efficiency in mammalian cells using various fluorescence-based methods Upon transfection of ARCA EGFP mRNA into the cytoplasm cells will express EGFP able to be characterized by strong bright green fluorescence upon excitation with excitation/emission maxima at 488 nm/509 nm respectively This product is provided as an mRNA capped at its 5 end and polyadenylated at its 3 end mimicking fully processed eukaryotic mRNA Advantages of mRNA transfection 1)No nuclear entry required mRNA directly translates to protein upon entry into the cytoplasm2)Faster protein expression compared to DNA transfection3)Promoter-independent protein expression4)No genomic integration risk5)Ideal for transfecting slowly dividing or non-dividing cells6)Protein expression directly correlates with the amount of transfected mRNA7)Transient expression protein is expressed within a limited timeframe
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Biotin-16-UTP is a biotin-labeled uridine triphosphate used for RNA detection and purification The biotin label allows RNA molecules to bind with streptavidin or anti-biotin proteins facilitating specific RNA detection and purification In in vitro transcription reactions Biotin-16-UTP can be incorporated into RNA molecules generating biotin-labeled RNA This labeled RNA has wide applications in RNA-protein interaction studies RNA detection and RNA purification Additionally biotin-labeled RNA can be applied in in vivo experiments to investigate RNA localization and function within cells
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Digoxigenin-11-UTP is a modified nucleoside triphosphate used for labeling RNA probes It incorporates a digoxigenin label attached to uridine triphosphate (UTP) allowing it to be incorporated into RNA molecules during in vitro transcription reactions Digoxigenin is a non-radioactive label widely utilized in molecular biology experiments especially in situ hybridization (ISH) and Northern blotting RNA probes labeled with Digoxigenin-11-UTP exhibit high sensitivity and low background noise providing clear detection results Additionally the digoxigenin label binds specifically to anti-digoxigenin antibodies making the detection process both straightforward and highly efficient Digoxigenin-11-UTP serves as an essential tool for studying gene expression and RNA localization
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A riboside form of nicotinamide; a precursor of NAD+ and a source of vitamin B3; increases intracellular and mitochondrial NAD+ content in C2C12, Hepa1.6, and HEK293 cells in a concentration-dependent manner at concentrations ranging from 1-1,000 μM; decreases acetylation of FOXO1 and SOD2 but not tubulin; increases NAD+ levels in liver and skeletal muscle and prevents body weight gain in mice fed a high-fat diet when used at a dose of 400 mg/kg per day; increases NAD+ in the cerebral cortex and reduces cognitive deterioration in a transgenic mouse model of Alzheimer’s disease
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