Hi-throughput dUTP qPCR Lo-ROX Mix comprises a master mix containing Taq polymerase, reaction buffer, MgCl2 and optimized dNTP/dUTP mix, PCR enhancers and a passive reference dye. It is designed for stringent amplification of highly-structured and very low copy-number DNA targets, preventing carry-over qPCR contamination, makes Hi-throughput dUTP qPCR lo-ROX ideal for multiplex assays on open, automated, high-throughput instruments.