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Nucleotides are composed of a nitrogenous base covalently bound to a 5-carbon sugar molecule and one or more phosphate functional groups. They are the molecules that make up the structural units of DNA and RNA.
An amine-reactive fluorescent probe; has been used, linked to adenosine A1 receptor ligands, as a fluorescent probe to quantify ligand-receptor binding; ex/em = 630/650 nm, respectively
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Cyanine 3-AA-CTP is a fluorescently labeled cytidine triphosphate derivative conjugated with the fluorophore Cy3 During in vitro transcription reactions Cyanine 3-AA-CTP is enzymatically incorporated into RNA strands producing Cy3-labeled RNA molecules suitable for fluorescence-based analyses Due to Cy3 s high fluorescence intensity and photostability the labeledRNAs facilitate diverse studies such as fluorescence microscopy imaging RNA-protein interaction assays and sensitive RNA detection methodologies Consequently Cyanine 3-AA-CTP serves as an important reagent for investigating RNA biology and function
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