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Nucleotides are composed of a nitrogenous base covalently bound to a 5-carbon sugar molecule and one or more phosphate functional groups. They are the molecules that make up the structural units of DNA and RNA.
4-Thio-UTP is a nucleotide triphosphate analog in which the oxygen atom at the 4-position of uracil is substituted by a sulfur atom It can be incorporated into RNA molecules during in vitro transcription reactions producing RNAs containing 4-thiouridine modifications This modification provides unique advantages in studying protein interactions RNA modifications and RNA labeling Due to the presence of the sulfur atom RNA modified with 4-Thio-UTP can form cross-links with proteins upon ultraviolet (UV) irradiation facilitating the study of RNA-protein interactions Additionally 4-Thio-UTP can be employed in research on RNA folding and function as well as in studying the cellular localization and roles of RNA
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N1-Methyl-Pseudouridine-5'-Triphosphate is a modified NTP for incorporation into messenger RNAs (mRNA) using T7 RNA Polymerase. Incorporation of N1-methylpseudouridine can reduce the immunogenicity of the resulting mRNA.
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