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Nucleotides are composed of a nitrogenous base covalently bound to a 5-carbon sugar molecule and one or more phosphate functional groups. They are the molecules that make up the structural units of DNA and RNA.
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7-deaza-dGTP (7-deaza-2-deoxyguanosine 5-triphosphate) contains a 5 mM solution of 7-deaza-dGTP as a dilithium salt. Nucleotide concentration is determined by measurements of absorbance at 257 nm. Supplied in: Milli-Q water as a lithium salt at (pH 7.0).
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Small and Specialty Supplier Partner Small and/or specialty supplier based on Federal laws and SBA requirements. Learn More
ARCA EGFP mRNA is a reporter mRNA suitable as a control reagent for studying transfection and expression efficiency in mammalian cells using various fluorescence-based methods Upon transfection of ARCA EGFP mRNA into the cytoplasm cells will express EGFP able to be characterized by strong bright green fluorescence upon excitation with excitation/emission maxima at 488 nm/509 nm respectively This product is provided as an mRNA capped at its 5 end and polyadenylated at its 3 end mimicking fully processed eukaryotic mRNA Advantages of mRNA transfection 1)No nuclear entry required mRNA directly translates to protein upon entry into the cytoplasm2)Faster protein expression compared to DNA transfection3)Promoter-independent protein expression4)No genomic integration risk5)Ideal for transfecting slowly dividing or non-dividing cells6)Protein expression directly correlates with the amount of transfected mRNA7)Transient expression protein is expressed within a limited timeframe
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