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Sequenced positive and negative RNA controls and RNA control mixes collected from various organisms. Products include mRNA, sgRNA, tracrRNA, and crRNA and are ideal for use in gene expression experiments.
Firefly Luciferase mRNA (ARCA 5mCTP UTP) encodes firefly luciferase protein originally isolated from Photinus pyralis Firefly luciferase is a widely used bioluminescence reporter molecule for studies on gene regulation cell viability ATP levels and in vivo imaging In the presence of ATP and oxygen luciferase catalyzes the oxidation of D-Luciferin (Cat C3654) emitting chemiluminescence at 560 nm This product is a 5 -capped 3 -poly(A)-tailed mRNA modified with 5mCTP and UTP mimicking mature eukaryotic mRNA Capped with ARCA (anti-reverse cap analog Cat B8175) via co-transcription it forms a Cap 0 structure enhancing stability and translation efficiency [1 2] The 5mCTP (Cat B7967) and UTP (Pseudo-UTP Cat B7972) modifications reduce innate immune activation and increase mRNA stability The poly(A) tail boosts stability and translation initiation efficiency
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