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TCS Antibody Purification Kit (ab109207) has been designed for tissue culture supernatant purification, and can be used to purify 10-50 mL in each purification.
Fatty Acyl-CoAs are a combination of Coenzyme A covalently bound to long-chain fatty acids. Fatty acyl-CoAs serve as both a precursor for triglyceride and phospholipids synthesis and a product of lipid catabolism. Catabolically, they facilitate the transport of fatty acids to the mitochondria for shuttling via the carnitine shuttle and subsequent β-oxidation of the fatty acid. Conversion of fatty acids to fatty acyl-CoAs is necessary for the generation of ATP during periods of high energy demand. Elevated fatty acyl-CoA levels may indicate dysregulation of lipid metabolism such as due to insulin resistance. Certain genetic disorders may also affect fatty acyl-CoA levels. BioAssay Systems’ fluorimetric fatty acyl-CoA assay works using a combination of enzymes that utilize fatty acyl-CoA as a substrate, which is coupled to form a fluorescent product. The resulting fluorescence intensity at λexc/em = 530/585 nm is linear to the fatty acyl-CoA concentration in the sample.
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Fluorescence antibody diluent is a modified PBS formulation with protein carrier and preservative. This ready-to-use modified PBS is formulated to deliver superior performance and signal preservation. The diluent is protease free and stabilizes fluorescent dyes for up to one month in a prediluted format.
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The conjugated goat anti-mouse polymer-alkaline phosphatase (AP) secondary antibody reacts with heavy and light chains on mouse IgG. It reacts with all mouse IgG subclasses and with mouse IgM. The innovative AP-polymerization technology provides a significant increase in staining sensitivity when compared to other conventional AP-conjugated secondary antibodies. Avidin-biotin blocking procedures are not necessary when using the MACH 2 conjugated secondary antibodies. The overall staining procedure uses one less reagent (streptavidin peroxidase) and rinsing step, eliminates avidin-biotin blocking procedures, and may reduce overall staining time. It can be used manually and on automated stainers.
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Ovalbumin (OVA) mRNA encodes a egg white glycoprotein with a molecular weight of approximately 45 000 daltons It can induce moderate immunogenicity in organisms and is commonly used as an immunogen in immunological experiments such as establishing animal models for hypersensitivity asthma etc EZ Cap OVA mRNA (5-moUTP) is provided at 1 mg/mL capped by co-transcriptional capping with a Cap 1 structure and polyadenylated Modified 5-moUTP incorporation reduces the host cell immune response and enhances mRNA stability and translation efficiency
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