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Various reagents for use in performing hapten-carrier conjugation reactions to yield immunogens that can be administered as immunization injections to host animals. Products include sulfhydryl-reactive forms of keyhole limpet hemocyanin and other proteins.
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Hemoglobin A0 is prepared from lysed human blood cells by sequential ion-exchange It is a mixture of non-glycated and glycated hemoglobin A0 fractions but is essentially free of HbA1a HbA1b HbA1c and HbF The protein is validated by HPIEC methods and is suitable as a primary standard in chromatographic or immunologic analyses The reported concentration is determined by Drabkins assay The material is supplied in liquid form in dilute potassium cyanide-phosphate buffer The concentration varies with manufacturing lot but the protein may be used directly by dilution in buffer in most experimental methods It is shipped frozen at -20oCShort term storage should be at 4oC Long term storage should be at -60oC Repeated cycles of freezing and thawing should be avoided
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This IMDM differs from the original formula because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.
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