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Competent Cells

Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
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61 – 75 of 402 results
61

Invitrogen™ MultiShot™ FlexPlate OmniMAX™ 2 T1R Competent Cells

MultiShot FlexPlate OmniMAX 2 T1R chemically competent E. coli cells are a superior strain of cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity.

ENCOMPASS_PREFERRED
62

Invitrogen™ One Shot™ TOP10F' Chemically Competent E. coli

One Shot TOP10F' Chemically Competent E. coli are identical to TOP10 cells, with the addition of an F' episome.

ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
Blue/White Screening Yes (lacZΔM15)
Cloning Unstable DNA Not suitable for cloning unstable DNA
Cloning Methylated DNA Yes (mcrA)
Transformation Efficiency Level High Efficiency (>1 x 109 cfu/μg)
High-throughput Compatibility Low
Improves Plasmid Quality Yes (endA1)
Promoter Trc, Tac, Lac
Preparing Unmethylated DNA No
Reduces Recombination Yes (recA1)
T1 Phage - Resistant (tonA) No
Antibiotic Resistance Bacterial Yes (Streptamycin, Tetracycline)
Format Tube
Product Type Chemically Competent Cells
Species E. coli (K12)
Product Line One Shot
Contains F' Episome Yes
63

MilliporeSigma™ Novagen™ HMS174 Competent Cell Set

HMS174 strains provide the recA mutation in a K-12 background.

64

Invitrogen™ ElectroMAX™ DH10B T1 Phage-Resistant Competent Cells

ElectroMAX DH10B T1 Phage-Resistant Competent Cells offer transformation efficiencies that are among the highest we offer and can exceed 1 x 1010 cfu/μg plasmid DNA in efficiency.

ENCOMPASS_PREFERRED
65

Invitrogen™ MAX Efficiency™ DH5α Competent Cells

MAX Efficiency™ DH5α Competent Cells are a well-known, versatile strain that is used in many everyday cloning applications. Prepared by a proprietary modification of the Hanahan method, these cells are suitable for high efficiency transformations in applications where low amount of DNA is used or to construct libraries using plasmid-derived vectors.

ENCOMPASS_PREFERRED
66

MilliporeSigma™ Novagen™ HT96™ NovaBlue Competent Cells

High-efficiency competent cells predispensed in 96-well plate for high-throughput applications

Content And Storage Store HT96 Competent Cells, SOC Medium and Test Plasmid at -70°C or below. Store Cap Strips, Plate Lids and Reagent Reservoirs at room temperature
Efficiency > 1 x 108CFU/μg
For Use With (Application) For high-throughput transformation
67

MilliporeSigma™ Novagen™ Origami™ 2 Competent Cells

Origami 2 strains have mutations in glutathione reducatase (gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation.

Content And Storage ≤-70°C
Efficiency Guaranteed efficiency: >2 x 106 cfu/μg
68

MilliporeSigma™ Novagen™ HMS174 (DE3) Competent Cells

HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.

Content And Storage ≤-70°C
Efficiency Guaranteed efficiency: >5 x 106 cfu/μg
69

MilliporeSigma™ Novagen™ Rosetta™ Blue (DE3) Competent Cells

Rosetta-gami B strains combine the key features of BL21, Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds.

Content And Storage ≤-70°C
Efficiency Guaranteed efficiency: >1 x 108 cfu/μg
70

Gibco™ CD Hybridoma AGT™ Medium

Animal origin-free, chemically defined, protein-free medium

ENCOMPASS
71

MilliporeSigma™ Novagen™ Rosetta-gami™ B Competent Cells

Rosetta-gami B strains combine the key features of BL21, Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds.

Content And Storage ≤-70°C
72

MilliporeSigma™ Novagen™ Rosetta-gami™ B (DE3) pLysS Competent Cells

Rosetta-gami B strains combines the key features of BL21, Origami, and Rosetta to enhances the expression of eukaryotic proteins. T7 lysozyme expression suppresses basal T7 expression.

Content And Storage ≤-70°C
Efficiency Guaranteed efficiency: >1 x 106 cfu/μg
73

MilliporeSigma™ Novagen™ Rosetta™ (DE3) pLacI Competent Cells

Rosetta strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. Contains the pLacI plasmid producing extra Lac repressor.

Content And Storage ≤-70°C
74

MilliporeSigma™ Novagen™ NovaBlue GigaSingles™ Competent Cells
GSA_VA

NovaBlue GigaSingles™ Competent Cells produce greater than 1 x 10⁹ colonies/μg plasmid DNA for cloning applications requiring high-efficiency transformations.

Content And Storage ≤-70°C
Efficiency Guaranteed efficiency: >1 x 109 cfu/μg