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MilliporeSigma™ Novagen™ Rosetta™ 2 (DE3) Competent Cells

Catalog No. 713974 Shop All MilliporeSigma Products
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71-397-4 1 mL
71-397-3 0.4 mL
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Catalog No. 71-397-4 Supplier MilliporeSigma™ Supplier No. 713974
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Description

Novagen's Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. Coli.

Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for 7 rare codones (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) on a compatible chloramphenicol-resistant plasmid. The tRNA genes are driven by their native promoters. DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
TRUSTED_SUSTAINABILITY

Specifications

Efficiency Guaranteed efficiency: >2 x 106 cfu/μg
Content And Storage ≤-70°C
Quantity 1 mL
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