Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
One Shot TOP10 Chemically Competent E. coli are ideal for high-efficiency cloning and plasmid DNA propagation and are provided at a transformation efficiency of 1 x 109 cfu/μg plasmid DNA.
Fisher Scientific Edge Order by 2 p.m. for same-day shipping. Learn More
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More
One Shot BL21 Star (DE3) Chemically Competent E. coli are designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7-promoter-based expression systems such as Champion pET vectors.
Fisher Scientific Edge Order by 2 p.m. for same-day shipping. Learn More
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More
High efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently.
Fisher Scientific Edge Order by 2 p.m. for same-day shipping. Learn More
One Shot TOP10 Electrocomp E. coli cells are ideal for high-efficiency cloning and plasmid propagation. Provided at a transformation efficiency of >1 x 1010 cfu/ μg supercoiled DNA, these cells allow for stable replication of high copy number plasmids.
Fisher Scientific Edge Order by 2 p.m. for same-day shipping. Learn More
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More
Suitable for the expression of non-toxic heterologous genes. The strain contains the lambda DE3 prophage that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG.
Fisher Scientific Edge Order by 2 p.m. for same-day shipping. Learn More
MultiShot StripWell BL21 Star (DE3) chemically competent E. coli cells are cloning competent cells designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7 promoter-based expression systems.
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More
Chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries.
Subcloning Efficiency DH5α Competent Cells are a versatile, chemically competent strain for cloning that provides a transformation efficiency of >1 x 106 cfu/μg plasmid DNA.
Fisher Scientific Edge Order by 2 p.m. for same-day shipping. Learn More
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More
One Shot BL21-AI Chemically Competent E. coli cells are designed for applications that require tight regulation and strong expression of toxic proteins from any T7-promoter-based expression systems.
Fisher Scientific Edge Order by 2 p.m. for same-day shipping. Learn More
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More
One Shot BL21 Star (DE3)pLysS Chemically Competent E. coli are cells designed for applications that require high-level expression of non-toxic recombinant proteins from low or high copy number vectors, such as pRSET and pET.
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More
MAX Efficiency™ DH5α Competent Cells are a well-known, versatile strain that is used in many everyday cloning applications. Prepared by a proprietary modification of the Hanahan method, these cells are suitable for high efficiency transformations in applications where low amount of DNA is used or to construct libraries using plasmid-derived vectors.
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More