Crosslinking, Labeling and Protein Modification
Invitrogen™ Alexa Fluor™ 633 C5 Maleimide
Alexa Fluor™ 633 is a bright, red fluorescent dye with excitation ideally suited to the 633 nm laser line.
Thermo Scientific™ PEG4-SPDP (PEGylated, long-chain SPDP crosslinker)
Long (25.7A) amine-to-sulfhydryl crosslinker; NHS-ester and pyridyldithiol groups at ends of 4-unit polyethylene glycol spacer arm; forms cleavable disulfide bonds; water-soluble.
Thermo Scientific™ EZ-Link™ Alkoxyamine-PEG4-Biotin
Biotinylate glycoproteins (aldehydes of oxidized sugars) with a mid-length (27.0A), pegylated biotinylation reagent containing a 4-unit PEG spacer for length and solubility.
Thermo Scientific™ EZ-Link™ NHS-PEG4 Biotinylation Kit
Biotinylate protein amines and maximize solubility of proteins with this long (29.0A) NHS-ester biotinylation reagent containing a pegylated, water-soluble spacer arm.
Thermo Scientific™ SMPH (Succinimidyl 6-((beta-maleimidopropionamido)hexanoate))
Crosslink amines to sulfhydryls with this long crosslinker composed of NHS-ester and maleimide groups at ends of an aliphatic, 12-atom (14.2A) spacer arm.
Thermo Scientific™ Pierce™ MMTS (methyl methanethiosulfonate)
Methyl methanethiosulfonate (CAS 2949-92-0); reversibly blocks cysteines (sulfhydryl groups) to facilitate study of enzyme activation and protein functions.
Thermo Scientific™ L-Photo-Methionine
Diazirine analogs of leucine and methionine to express proteins in cell culture that will crosslink their protein interactors upon UV-light activation in vivo.
Thermo Scientific™ SDA (NHS-Diazirine) (succinimidyl 4,4'-azipentanoate)
Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).
Thermo Scientific™ Sulfo-SDAD (Sulfo-NHS-SS-Diazirine) (sulfosuccinimidyl 2-((4,4'-azipentanamido)ethyl)-1,3'-dithiopropionate)
Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).
Thermo Scientific™ MS(PEG)24 Methyl-PEG-NHS-Ester Reagent
Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units).
Thermo Scientific™ EZ-Link™ Psoralen-PEG3-Biotin
Biotinylate DNA or RNA via intercalation and conjugation with thymine- and other pyrimidine-containing bases with this light-activated biotinylation reagent.
Thermo Scientific™ DyLight™ 350 NHS Ester
Label antibodies or other proteins as probes for fluorescence imaging assays with the full spectral range of NHS ester-activated DyLight Dyes.
Thermo Scientific™ NHS-Fluorescein (5/6-carboxyfluorescein succinimidyl ester), mixed isomer
Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).