Protein Labeling Reagents
Dojindo Molecular Technologies Inc Biotin Labeling Kit-NH2 (1 mg), is used for preparation of biotin-labeled IgG for enzyme immunoassay (EIA). It only requires one hour to have high recovery of conjugates, applicable for 1mg IgG.
Biotin Labeling Kit-NH2 is mainly used for the preparation of biotin-labeled IgG for enzyme immunoassay (EIA), NH2-reactive biotin, a component of this kit, has succinimidyl groups (NHS) that react with amino groups on proteins or other molecules. The labeling process is very simple, just add the NH2- reactive Biotin to IgG solution(1 mg protein/sample) and incubate at 37°C for 10 minutes. An average of 5 to 8 biotin molecules conjugate to each IgG molecule. The number of biotin molecules per protein can be determined by HABA assay. Excess biotin molecules can be removed by a filtration tube. The molecular weight of the protein to be labeled with this kit should be greater than 50,000. This kit contains all of the necessary reagents for labeling, including the storage buffer for conjugates. Dojindo has various protein labeling reagents. (https://www.dojindo.com/labeling-chemistry/)
Revvity Health Sciences Inc PhenoVue Cell Painting Kit 1 x 384-wells
PhenoVue Cell Painting Kit 1 x 384-wells
Revvity Health Sciences Inc IVISense Gastrointestinal 750 Fluorescent Probe (GastroSense)
IVISense Gastrointestinal 750 Fluorescent Probe (GastroSense)
Sartorius Incucyte Mouse IgG1
Incucyte Mouse IgG1 Fabfluor Antibody Labeling Dye is designed for quick easy labeling of Fc containing test antibodies with a green or red fluorophore. Once labeled the Fabfluor-antibody complex can be used for identification of surface expressed antigens in live cells. In the absence of expressed specific antigen little or no signal is seen on the cells.With the Incucyte integrated analysis software background fluorescence is minimized. This reagent has been validated for use with a number of different antibodies in a range of cell types.
AURORA BIOLABS LLC Biotin Protein Ligase (BirA), 100 μg
Biotin-protein ligase activates biotin to form biotinyl 5' adenylate and transfers the biotin to biotin-accepting proteins. Biotin ligase also controls biotin synthesis in Escherichia coli. As a homodimer, it negatively controls the biotin synthetic operon.
Dojindo Molecular Technologies Inc DALGreen – Autophagy Detection (20 nmol)
DALGreen is used to detect autophagy in live cells. Autophagy is an intracellular degradation system, where dysfunctional proteins and organelles are degraded. In this process, aggregated dysfunctional proteins are surrounded by the double membrane to form an autophagosome. DALGreen, which is a small hydrophobic molecule, passes the plasma membrane of live cells and is incorporated in the autophagosome. After lysosomes fuse with the autophagosome, the environment in the autolysosome becomes acidic. DALGreen fluoresces stronger as acidity increases. The quality of this dye enables live-cell imaging with fluorescence microscopy and quantitative assay by flow cytometry. Excitation and emission spectra of DALGreen: (λex: 405 nm, λem: 525 nm). For monitoring autophagosomes, we recommend DAPGreen [D676] and DAPRed [D677]. Dojindo offers various reagents for autophagy research. (https://www.dojindo.com/autophagy-research/#jmp-b).
Cell Signaling Technology Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Alexa Fluor 647 Conjugate) 100 µl
Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Alexa Fluor 647 Conjugate) 100 µl
Medchemexpress LLC Sulfo-NHS-LC-biotin sodium | 191671-46-2 | MFCD00078534 | 98.0% | 556.59 | C20H29N4NaO9S2 | 10 MG
Sulfo-NHS-LC-Biotin sodium is a water-soluble, amine-reactive sulfo-NHS ester of biotin with a long-chain spacer (LC) that enables efficient labeling of primary amines on proteins and other biomolecules. It is formulated as the sodium salt to improve aqueous solubility and is suited for selective cell-surface biotinylation, detection, and affinity capture applications.
- Amine-reactive sulfo-NHS ester for efficient biotinylation.
- Long-chain spacer reduces steric hindrance and preserves biomolecule function.
- Water-soluble sodium salt suitable for aqueous labeling of surface proteins.
- Limited membrane permeability for selective cell-surface labeling.
- High purity and well-characterized for reproducible results.
- Soluble in DMSO (approx. 175 mg/mL); store sealed and away from moisture.
System Biosciences LLC ExoGlow-Protein EV Labeling Kit (Green), 20 Reactions
Label EV proteins green (511 nm excitation/525 nm emission) with a reagent specifically optimized for EV labeling.