Protein Labeling Reagents
Biotium Cyanine 647 Succinimidyl Ester
Biotium offers high quality amine-reactive succinimidyl ester (SE, or NHS-Ester) dyes used to label proteins, oligonucleotides, and nucleic acids. We offer Cyanine 555 (Cy3), Cyanine 647 (Cy5), Cyanine 770 (Cy5.5), and Cyanine 750 (Cy7) as high quality succinimidyl esters. Also see our CF Dye Succinimidyl Ester, available with more than 20 bright and photostable CF Dyes. Cyanine 647 is structurally identical to Cy5 from GE Healthcare. Properties: Ex / Em (water) = 650/665 nm; Dark blue solid soluble in DMF and DMSO; Store at -20°C and protect from moisture and light; C 43 H 58 N 4 O 10 S 2; MW: 855. Cy3, Cy5, Cy5.5, and Cy7 are registered trademarks of GE Healthcare.
Biotium Mix-n-Stain™ CF594 Small Ligand Labeling Kit
Mix-n-Stain™ Small Ligand Labeling Kits are designed for rapid labeling of low molecular weight (MW 150 -5000) ligands without a purification step. Suitable ligands or substrates include SNAP-tag, CLIP-tag™ and HaloTag ligand derivatives that have an aliphatic amine. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Deep-red fluorescent CF594 dye has excitation/emission at 593/614 nm. It is suitable for labeling ligands for cell surface staining.
Biotium CF568 Biocytin
Fluorescent biocytin conjugates are cell-impermeant, fixable polar tracers with biotin and an aldehyde-fixable primary amine groups. Polar tracers are commonly used to investigate cell-cell and cell-liposome fusion as well as membrane permeability and transport through gap junctions or cell uptake during pinocytosis. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Red fluorescent CF568 dye has excitation/emission at 562/583 nm.
Biotium CF770R Succinimidyl Ester
CF Dye Succinimidyl Esters (SE, also known as NHS esters) are amine-reactive fluorescent dyes. SE dyes are commonly used to label antibodies and other proteins on free amine groups, such as lysine residues. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red/near-IR fluorescent CF770R dye has excitation/emission at 770/701 nm.
Biotium MitoView™ 720, 1000X in DMSO
MitoView™ 720 is a near-infrared live cell mitochondrial dye with absorbance/emission at 720/758 nm. While optimally detected using Cy7 settings, the dye is bright enough to be imaged in the Cy5 channel.
STEMCELL Technologies GloCell™ Fixable Viability Dye Violet 540, Size: 100 Tests
GloCell™ Fixable Viability Dye Violet 540 is a fluorescent cell viability dye for staining live/dead mammalian cells in applications such as flow cytometry. The dye irreversibly binds intracellular and cell surface amine groups and can be utilized prior to fixation, permeabilization, or cryopreservation. Cells with compromised plasma membranes become permeable to the GloCell™ dye, resulting in a greater fluorescence compared to live cells in a cell viability assay.GloCell™ Fixable Viability Dye Violet 540 is excited by the violet laser at 405 nm and it has a fluorescence emission maximum of 540 nm that can be detected using the 525/50 band pass filter used to detect AmCyan.
Biotium ViaFluor 405 Live Cell Microtubule Stain, Trial Size
ViaFluor™ 405 is a blue fluorescent taxol probe with excitation/emission at 408/452 nm that allows fluorescent staining of microtubules in living cells for detection in the DAPI channel. Supplied at 1000X in DMSO, with a vial of 100 mM verapamil, which can enhance staining in some cell types.
Biotium 5(6)-Tetramethylrhodamine Maleimide
5(6)-Carboxytetramethylrhodamine maleimide has C6-linker that enhances the fluorescence after the dye is conjugated to proteins. The product is a direct replacement for 5(6)-carboxytetramethylrhodamine C5-maleimide (91040).Properties: Ex/Em (MeOH) = 540/565 nm; Red solid soluble in DMF or DMSO; Store at -20°C and protect from light, especially in solution; C35H36N4O6; MW: 608.66.
Biotium Cyanine 555 Tyramide
Cyanine 555 tyramide (Ex/Em 555/565 nm) can be used for tyramide signal amplification (TSA), which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ. Cyanine 555 is structurally identical to Cy3 from GE Healthcare.
STEMCELL Technologies GloCell™ Fixable Viability Dye Red 780, Size: 100 Tests
GloCell™ Fixable Viability Dye Red 780 is a fluorescent cell viability dye for staining live/dead mammalian cells in applications such as flow cytometry. The dye irreversibly binds intracellular and cell surface amine groups and can be utilized prior to fixation, permeabilization, or cryopreservation. Cells with compromised plasma membranes become permeable to the GloCell™ dye, resulting in a greater fluorescence compared to live cells in a cell viability assay.GloCell™ Fixable Viability Dye Red 780 is excited by the red laser at 633 nm and it has a fluorescence emission maximum of 780 nm that can be detected using the 780/60 band pass filter used to detect APC-Cy7.
Abcam NBD- x , SE, 25MG
MW 391.34 Da, Purity >90%. Lipophilic green fluorescent probe for labeling antibodies and proteins. X-spacer provides separation between dye and protein for increased sensitivity and reduced impact on protein function. Functional analog of the dinitrophenol (DNP) hapten. Fluorescence is quenched when bound by anti-DNP antibodies.
The product is subject to the following: Abcam Restricted Use Statement
Aat Bioquest 5-TAMRA, SE [5-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-68-7*
The single TAMRA isomers are increasingly preferred for labeling peptides and nucleotides because they give better resolution in HPLC purification that is often required in the conjugation processes.
Biotium Phalloidin, CF568, 50 U
Phalloidin conjugates are actin filament stains for fixed and permeabilized cells. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Red fluorescent CF568 has excitation/emission at 562/583 nm.
Biotium CF568 Hydrazide
Our CF dye hydrazides are bright, extremely water-soluble and nontoxic. These properties make the dyes excellent fixable polar tracers for microinjection into cells. Hydrazides also can be used for labeling aldehyde or ketone groups, however, for this application we recommend using the more reactive CF dye aminooxy forms. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Red fluorescent CF568 dye has excitation/emission at 562/583 nm.