Protein Modification Reagents
Dojindo Molecular Technologies Inc FeBABE (1MG), 1-(p-Bromoacetamidobenzyl) ethylenediamine N,N,N EN E/em>-tetraacetic acid, acid iron(III)
Bromoacetamidobenzyl-EDTA (BABE) is a chelate labeling reagent that conjugates with sulfhydryl groups. The iron chelate of BABE (FeBABE) is a unique tool for determining the three-dimensional structure of proteins and the binding structures of protein-protein or protein-DNA complexes. BABE adds EDTA moieties to proteins through their sulfhydryl groups. Once attached to a protein, FeBABE cuts a nearby peptide or DNA chain. The cleavage site is within 12 angstroms of the FeBABE binding site. Iron (II)-chelate cleaves a peptide or DNA chain in the presence of hydrogen peroxide. The cleavage reaction completes quickly: 10 seconds to 20 minutes of incubation is sufficient. The size of the cleaved fragment is analyzed with gel electrophoresis such as SDS-PAGE.
Sigma Aldrich Fine Chemicals Biosciences Pepsin from porcine gastric mucosa | 9001-75-6 | MFCD00081840 | 5g
Pepsin from porcine gastric mucosa | Mol Wt: 35 kDa | 9001-75-6 | MFCD00081840 | 5g
Worthington Biochemical Corporation COLLAGENASE IV /5GM
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity. Suggested for pancreatic islet primary isolation. A dialyzed, lyophilized powder.
Worthington Biochemical Corporation COLLAGENESE TYPE 1 FILTERED
Collagenase, Type 1 (Code: CLS-1), Filtered Through a 0.22 Micron Membrane and Lyophilized in Vials to Contain 50mg per Vial, Store at 2 deg. to 8 deg.C, Source: Clostridium histolyticum, Minimum Activity: 125 units/mg Dry Weight, 50mg
Genscript Corporation TEV Protease, His
Tobacco Etch Virus Protease is a highly site-specific cysteine protease that is found in the tags from fusion proteins. The optimal temperature for cleavage is 30°C; also it can be used at temperature as low as 4°C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of recombinant viral TEV protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.The optimum recognition site for this enzyme is the sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) [ENLYFQ(G/S)] and cleavage occurs between the Gln and Gly/Ser residues, The most commonly used sequence is ENLYFQG. The protease is used to cleave affinity tags from fusion proteins. The optimal temperature for cleavage is 30°C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of recombinant viral TEV protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.
AURORA BIOLABS LLC PreScission Protease (HRV 3C), 1000 units
PreScission Protease is a fusion protein of glutathione S-transferase (GST) and human rhinovirus (HRV) type 14 3C protease. The protease specifically recognizes a subset of sequences which include the core amino acid sequence Leu-Phe-Gln/Gly-Pro cleaving between the Gln and Gly residues. Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the secondary and tertiary structures of the fusion protein as well.
Sigma Aldrich Fine Chemicals Biosciences Collagenase P from Clostridium histolyticum |
Collagenase P from Clostridium histolyticum |
Aat Bioquest SDA alkyne, 1mg .
SDA alkyne contains both diazirine photoaffinity moiety and alkyne functional group. It is an excellent building block to introduce photoaffinity function into an azide-modified biomolecule via the well-known click chemistry. Diazirines are known for their ability to undergo photochemical reactions when exposed to ultraviolet (UV) light, specifically by forming highly reactive carbene intermediates that react with nearby molecules, forming covalent bonds. This property makes diazirines useful for studying protein-protein, protein-nucleic acid interactions, ligand-receptor binding, and other biomolecular interactions. It's important to note that diazirines are highly reactive and can be challenging to handle due to their instability. They require careful storage and handling, typically in a controlled environment.
Cole-Parmer White peg for modular stainless steel drying racks, 4"L
Removable snap-in plugs and pegs come in packs of six.
Worthington Biochemical Corporation PAPAIN 25MG
Supplied as a two times crystalline suspension in 50mM sodium acetate, pH 4.5. To insure full activity, the enzyme should be incubated in a solution containing 1.1mM EDTA, 0.067mM mercaptoethanol and 5.5mM cysteine-HCl for 30 minutes.