Protein Modification Reagents
Gibco™ PNGase F Glycan Cleavage Kit
PNGase F Glycan Cleavage Kit includes all components necessary to perform the enzymatic removal of almost all N-linked oligosaccharides from glycoproteins.
Thermo Scientific™ Guanidine-HCl
Pure, crystalline guanidium hydrochloride (GdnHCl, GuHCl), CAS 50-01-1; denaturant for protein biology methods (elution, solubilization, refolding, storage).
Invitrogen™ Tris-(2-Carboxyethyl)phosphine, Hydrochloride (TCEP)
Tris-(2-Carboxyethyl)phosphine, Hydrochloride (TCEP)
Thermo Scientific™ Urea
Pure, crystalline urea (CH4N2O), CAS 57-13-6; denaturant with low UV-absorptivity for protein biology methods (elution, solubilization, refolding, storage).
Thermo Scientific™ Pierce™ HRV 3C Protease Solution Kit (2 units/μL)
Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.
Thermo Scientific™ Pierce™ Hydroxylamine-HCl
NH2OH (CAS 7803-49-8) to cleave Asn-Gly peptide bonds or deprotect sulfhydryls following S-acetylthioacetate modification with SATA reagent.
Invitrogen™ DTNB; Ellman's Reagent, 5,5'-Dithiobis-(2-Nitrobenzoic Acid)
DTNB; Ellman's Reagent, 5,5'-Dithiobis-(2-Nitrobenzoic Acid)
Thermo Scientific™ MT(PEG)4 Methyl-PEG-Thiol Compound
MT(PEG)n: methyl and sulfhydryl compound with a 4-unit polyethylene glycol (PEG) spacer; methylate and pegylate quantum dots, gold surfaces, silver.
Invitrogen™ Thiol and Sulfide Quantitation Kit
Ultrasensitive colorimetric assay for quantifying both protein and nonprotein thiols
Thermo Scientific™ MM(PEG)24 Methyl-PEG-Maleimide Reagent
Pegylate sulfhydryls (-SH groups) on proteins (cysteine residues) or assay surfaces using MS(PEG); sulfhydryl-reactive (maleimide), PEG compounds (12 and 24 EG units).
Thermo Scientific™ CA(PEG)8 Carboxy-PEG-Amine Compound
CA(PEG)n: pegylated amino acids of the form carboxy-PEG-amine; with 4 to 24 polyethylene glycol units; for surface- and molecule-pegylation applications.
Thermo Scientific™ MA(PEG)12 Methyl-PEG-Amine Compound
MA(PEG)n: methyl- and amine-capped polyethylene glycol compounds containing 4 to 24 PEG units; use for surface-modification and molecule-pegylation applications.
Thermo Scientific™ Pierce™ Sulfhydryl Addition Kit
Modify protein primary amines to create free sulfhydryl groups for labeling, crosslinking or immobilization techniques; complete kit with SATA reagent and hydroxylamine.
Thermo Scientific™ CA(PEG)24 Carboxy-PEG-Amine Compound
CA(PEG)n: pegylated amino acids of the form carboxy-PEG-amine; with 4 to 24 polyethylene glycol units; for surface- and molecule-pegylation applications.