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Reporter genes are attached to a regulatory sequence of a gene of interest. When expressed by the host organism, they generate measurable, easily identifiable characteristics that can be used as an indication of whether a gene has been taken up or expressed.
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Complex of human CDK1 (Cyclin Dependent Kinase 1) and human Cyclin A2. The two constructs are: recombinant human CDK1, full length encompassing amino acids 2-297 (end) and recombinant human Cyclin A2, full length encompassing amino acids 2-432 (end). Both constructs contain an N-terminal GST-Tag. A short peptide linker placed between GST and the protein includes a PreScission protease cleavage site. The two proteins were co-expressed in Sf9 insect cells and spontaneously formed a complex, which was affinity purified.
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