Protein Mass Spectrometry Sample Prep
Promega MS Compatible Human Protein Extract, Intact
Mass spectrometry-compatible yeast and human protein extracts are designed specifically for mass spectrometry applications. The extracts are predigested and cleaned up by solid-phase extraction for immediate use in liquid chromatography/mass spec analysis
Promega MS Compatible Human Protein Extract, Digest
Mass spectrometry-compatible yeast and human protein extracts are designed specifically for mass spectrometry applications. The extracts are predigested and cleaned up by solid-phase extraction for immediate use in liquid chromatography/mass spec analysis
Promega Corporation Trypsin Platinum, Mass Spec Grade
Achieve superior autoproteolytic resistance with Recombinant Trypsin for most accurate biotherapeutic protein characterization and specialized proteomics applications. Free of chymotryptic activity, works with existing trypsin protocols, ensuring excellent lot-to-lot reproducibility and free of contaminating animal proteins.
Promega Corporation ProAlanase, Mass Spec Grade (0.2 μg/μL)
The new site-specific endoprotease targets proline and alanine residues, cleaving primarily C-terminal to these residues. Active at acidic pH (1-5.5) with a pH optimum of ~1.5, and offers short digestion times of 1-2 hours.
Protifi LLC Universal proteomics sample preparation kits, minis (100 µg - 300 µg) with solutions
S-Trap mini sample processing in a full kit format for samples from 100 µg - 300 µg. The kit includes S-Trap minis, extra tubes, and lysis, wash and digestion buffers. The user must supply HPLC or MS grade MeOH and their choice of protease.
Electron Microscopy Sciences Trypsin Kit Trypsin Kit Large
Trypsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin; mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Trypsin digestion improves immunoreactivity of some antigens in FFPE tissue sections. For cytokeratin clone AE3 and AE1/AE3, this enzyme works much better than boiling the tissue with citrate buffer.
Intended Use: Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC.
Reagent: This enzyme is supplied as concentrated form along with buffer. Reagent B (Buffer) is 100ml and Reagent E (Enzyme) is 10ml.
