Protein Mass Spectrometry Sample Prep
Waters Corp GlycoWorks RapiFluo-MS N-Glycan Eco Starter Kit, 96 Samples, UPLC Glycan BEH Column
The GlycoWorks RapiFluo-MS N-Glycan Eco Starter Kit offers a fast, sensitive method for released N-glycan analysis. It simplifies sample preparation to three steps in 30 minutes. Using RapiFluor-MS (RFMS) technology, it provides increased fluorescence and superior mass spectral response for characterization and monitoring with a single label. The “Eco” design replaces DMF with DMSO as a safer reagent solvent.
- Reduces N-glycan sample preparation to 3 easy steps in as little as 30 minutes.
- Provides increased fluorescence quantification and superior mass spectral response with RapiFluor-MS technology.
- Enables versatile labeling, using a single label for both characterization and routine monitoring.
- Replaces DMF with DMSO as a greener and safer reagent solvent, making it eco-friendly.
- Includes all necessary components for N-glycan analysis.
Waters Corp GlycoWorks RapiFluor-MS N-Glycan Starter Kit, 24 Samples, ACQUITY Premier UPLC Glycan BEH Column
This kit provides everything necessary for enzymatic deglycosylation, RapiFluor-MS labeling, SPE clean-up, and UPLC analysis, including a UPLC column and mobile phase concentrate. It offers a streamlined, fast, and highly sensitive method for analyzing released N-glycans.
- Three easy steps in as little as 30 minutes, reducing complicated sample preparation.
- RapiFluor-MS provides increased fluorescence quantification and supreme mass spectral response.
- Uses a single label for characterization to routine monitoring.
- Processes 24 samples in a 3×8 format.
- Includes a labeling module for the solubilization and labeling of released glycosylamine.
- Features a UPLC Glycan BEH Amide column, 1.7 μm, 2.1 × 150 mm.
Waters Corp WATERS CORPORATION RapiFluor-MS N-Glycan Kit, Automation, 96 Samples
The GlycoWorks RapiFluor-MS N-Glycan Kits for automation are designed for high-throughput glycoprotein sample preparation for N-glycan analysis with minimal user intervention. The protocol is validated for monoclonal antibodies and a wide range of other N-linked glycoproteins.
- Rapid sample preparation in three steps, under an hour.
- Enhanced fluorescence quantification and superior mass spectral response with RapiFluor-MS labeling.
- Single label for characterization and routine monitoring.
- Comprehensive kits for deglycosylation, labeling, and SPE cleanup.
- Reagents scaled for automated instruments, offering platform versatility.
- Includes script information to accelerate automation results.
- “Eco” version uses DMSO as the reagent solvent.
- HILIC μElution Plate for efficient contaminant removal.
Waters Corp WATERS CORPORATION GlycoWorks RapiFluor-MS N-Glycan Eco Starter Kit, 96 Samples
This kit is designed to provide scientists with all the necessary materials to successfully perform fast N-glycan deglycosylation, rapid fluorescent labeling, and glycan extraction with minimal preparation time. It uses optimized deglycosylation conditions and reagents, a novel rapid label called RapiFluor-MS for sensitive fluorescence and mass detection, and solid-phase extraction (SPE) for sample clean-up prior to LC analyses.
- Optimized deglycosylation conditions and reagents for fast release.
- Features RapiFluor-MS, a novel rapid label for sensitive fluorescence and mass detection.
- Sample clean-up via solid-phase extraction (SPE) with the HILIC μElution™ Plate.
- Streamlined protocol for fast sample preparation, minimizing handling and loss.
- Each reaction is optimized for the release, labeling, and extraction of N-glycans from 15 to 40 μg of glycoprotein.
Waters Corp Sample Preparation Kit, High Digest, Ambient Refill, 96 Analysis
This sample preparation workflow streamlines complex pre-analytic procedures for LC-MS protein quantification using the surrogate peptide technique. It is designed for high protein content samples (1.0 to 5.25 mg total protein per sample), directly degrading proteins in whole, non-immuno-purified plasma or serum. The kit aims to shorten method development time and provide repeatable, accurate, and sensitive protein measurement.
- Includes pre-measured, lot-traceable digestion buffer, trypsin dissolving reagent, digestion inactivation reagent, and a RapiGest denaturant vial.
- Designed for high protein content samples (1.0 to 5.25 mg total protein per sample).
- Supports both 3-step or 5-step protocols for protein quantification.
- Optimized for LC-MS/MS detection and RPLC separation.
Bioss Salmonellae sp.
Salmonellae sp Primary Antibody Unconjugated Host Species Mouse Target Species Clonality Monoclonal Applications ELISA Concentration 100 g/ml Size 100 ug Storage Buffer PBS (pH 7 2) with 0 1% azide
Cayman Chemical SP-A196-215humntrIfl 10mg
A peptide fragment of surfactant protein A; inhibits LPS-induced TNF-α release in JAWSII mouse dendritic cells at 1 and 10 µM; induces phagocytosis of P. aeruginosa by JAWSII cells at 75 µM; intratracheal administration decreases disease severity and lung colony forming units in a mouse model of P. aeruginosa infection at 50 UG/animal
Waters Corp N-Glycan Eco Starter Kit, Premier UPLC Glycan BEH Column, 24 Samples
This starter kit offers a streamlined, fast, and highly sensitive method for analyzing released N-glycans. It reduces complex sample preparation to three easy steps in as little as 30 minutes. The kit includes components for deglycosylation, labeling, clean-up, sample collection, a UPLC Glycan BEH Amide Column, and Mobile-Phase Concentrate for 24 samples.
- Streamlined, fast, and highly sensitive analysis of released N-glycans.
- Sample preparation reduced to 3 easy steps in as little as 30 minutes.
- RapiFluor-MS provides increased fluorescence quantification and supreme mass spectral response.
- Single label for characterization and routine monitoring.
- Kit contains materials to process 24 samples in a 3x8 format.
Waters Corporation Labeling Module, RapiFluor-MS, Automation, GlycoWorks, 48 or 96 Samples
The GlycoWorks RapiFluor-MS Labeling Module-Automation provides all the components needed for RapiFluor-MS labeling for automation platforms. It is designed for quick enzymatic release and labeling of N-glycans and can process 48 or 96 samples at a time, allowing for high-throughput glycoprotein sample preparation for N-glycan analysis with minimal human participation.
- Provides all components for RapiFluor-MS labeling on automation platforms, enabling quick enzymatic release and labeling of N-glycans.
- Processes 48 or 96 samples for high-throughput glycoprotein preparation.
- Features a fast label for sensitive fluorescence and strong mass detection.
- Includes two 55 mg vials of RapiFluor-MS reagent and two 1 mL vials of reagent solvent.
- Contains hazardous material, requiring special freight handling.
Waters Corp GlycoWorks RapiFluor-MS N-Glycan Script Parameters Starter Kit, Automation, 96 Analyses, HILIC
The GlycoWorks RapiFluor-MS N-Glycan Script Parameters Starter Kit – Automation enables high-throughput glycoprotein sample preparation for N-glycan analysis on automated platforms. Its protocol is validated for monoclonal antibodies and diverse N-linked glycoproteins, with scaled reagents for versatile automated instrumentation.
- Includes script parameters guideline and liquid classes table to accelerate automated script development.
- Contains RFMS labeled standard, column, and mobile phase concentrate for RapiFluor-MS Library compatibility.
- Supports 96 analyses, featuring HILIC separation and MS, HRMS, MS/MS, UV detection methods.
- Components requiring cold chain shipping (deglycosylation module, standards) are clearly designated.
- Optimized for LC-MS systems in glycan applications.
Waters Corp ProteinWorks eXpress Digest Kit, LC-MS Protein Quantification, 96 Analysis
The ProteinWorks eXpress Digest Start-up Kit is designed to simplify and accelerate the direct digestion of immunopurified plasma & serum samples for LC-MS protein quantification using the surrogate peptide approach.
- Simplifies and accelerates direct digestion for LC-MS protein quantification of immunopurified plasma & serum samples.
- Provides a standardized, proven route for fast, accurate, and precise protein quantification in bioanalysis.
- Includes pre-measured, lot-traceable chemicals optimized for dependable LC-MS protein quantification.
- Enables rapid quantification, such as Infliximab down to 10 ng/mL.
- Achieves single-digit intra-kit and inter-kit reproducibility across multiple monoclonal antibodies.