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Reagents, kits, and instruments for use during the extraction of proteins from bacteria, cultured mammalian cells and tissues, insect cells, yeast cultures, flowering plants, etc. Products include lysis buffers, master mixes, and solubilizing solutions.
NativeLyser is a bacterial cell lysis buffer formulated to promote the efficient breakdown of bacterial cells while solubilizing and stabilizing the released recombinant proteins NativeLyser contains a proprietary mix of stabilizers that prevent the loss of recombinant proteins during the preparation of cell-free extracts By preventing denaturation proteolytic degradation and non-specific protein aggregation NativeLyser creates a protein-friendly environment when recombinant proteins are released from the bacterial cytoplasm We recommend the use of NativeLyser to purify proteins expressed using the NativeFolder Bacterial Culture Medium (Catalog number K1010004) NativeLyser is provided as a 5X concentrated solution (200 mL) that can be diluted with di-water to produce 1L of ready-to-use buffer For in vitro research use only
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