Bioluminescence Assays and Reagents
Amsbio LLC EF1a-Luciferase (firefly)-2A-GFP (Bsd)/ Concentrated Lentivirus
EF1a-Luciferase (firefly)-2A-GFP (Bsd) in vivo ready
BIOHIPPO h/m CD36-sh-GFP/Luc
h/m CD36-sh-GFP/Luc shRNA lentiviral vector expressing GFP/Luc with selection for studies in Human/Mouse Each vial provides a nominal titer of 2x10E6 transducing units TU Validated h/m CD36 shRNA Lentivirus High-titer shRNA lentiviral particles specific for human/mouse CD36 molecule (CD36) The shRNA has been validated to meet or exceed 70% CD36 knockdown efficiency using a specific fluorescence-based method that is more rapid and reliable than qPCR The shRNA lentivirus is ultra-purified and concentrated to high-titer by PEG precipitation and sucrose gradient centrifugation and ideal for transducing difficult-to-transfect cells including thawed and/or primary cells Order a shRNA lentivirus set and receive lentivirus produced from mix of 2 independent shRNAs validated to knockdown the target gene ( 70%) plus control lentivirus produced from mix of 2 scrambled shRNA constructs
BIOHIPPO CMV-Luc-RFP-BSD
CMV-Luc-RFP-BSD is a fluorescence and luciferase dual imaging reporter lentivirus based on the LFV-V1a vector layout under control of the CMV promoter containing a Luc-T2A-RFP-P2A-BSD expression cassette and Blasticidin resistance marker This construct co expresses luciferase and fluorescent reporter proteins to enable correlated bioluminescent and fluorescent readouts in the same cell population Each vial provides a nominal titer of 2x10E6 transducing units TU The lentiviral particles are supplied ready to use for stable gene delivery to mammalian cells and can facilitate generation of long term reporter cell lines They are well suited for in vitro cell based assays in vivo optical imaging high content screening and mechanism of action studies This product is intended for research use only and is not for diagnostic or therapeutic procedures