Bioluminescence Assays and Reagents
Revvity Health Sciences Inc sensilite Enhanced Flash Luminescence (Firefly Luciferase) Reporter Gene Assay System, 1,000 Assay Points
sensilite Enhanced Flash Luminescence (Firefly Luciferase) Reporter Gene Assay System, 1,000 Assay Points
BPS Bioscience Inc STAT3 Reporter Kit (STAT3 Signaling Pathway) | 500 Reactions
The STAT3 Reporter kit is designed for monitoring the activity of the STAT3 signaling pathway in cultured cells. The kit contains transfection-ready STAT3 luciferase reporter vector. This reporter contains a firefly luciferase gene under the control of STAT3-responsive element located upstream of a minimal promoter. The STAT3 reporter is premixed with constitutively expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity.
Sigma Aldrich Fine Chemicals Biosciences firefly Luciferase Assay f
firefly luciferase is widely used as a reporter for studying gene regulation and function and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues. firefly luciferase is a 62 000 Dalton protein which is active as a monomer and does not require subsequent processing for its activity. The enzyme catalyzes ATP-dependent D-luciferin oxidation to oxyluciferin producing light emission centered at 560 nm. Light emitted from the reaction is directly proportional to the number of luciferase enzyme molecules. The firefly Luciferase Assay is designed for simple and efficient quantitation of firefly luciferase reporter enzyme activity from cultured cells with high sensitivity and linearity. This is a flash-type luminescence assay that requires signal to be measured immediately after adding working SOLUTIONon to samples. The luminescence signal decays over the course of about 10 minutes of reaction
Cayman Chemical MyrIcetIn 3-galactosIde 10mg
A flavonoid with diverse biological activities; inhibits xanthine oxidase in a cell-free assay at 100 UG/ml; cytotoxic to K562 cells (IC50 = 220 UG/ml); inhibits hydrogen peroxide-induced MDA formation in K562 cells at 110, 220, and 440 UG/ml; reduces carrageenan-induced paw edema in wild-type mice at 0.26 and 0.78 mg/kg; reduces formalin-induced paw licking in mice
ABclonal Technology PEF1 Rabbit mAb
This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family The encoded protein has been shown to form a heterodimer with the programmed cell death 6 gene product and may modulate its function in Ca(2 ) signaling Alternative splicing results in multiple transcript variants and a pseudogene has been identified on chromosome 1
Revvity Health Sciences Inc steadylite plus Reporter Gene Assay System, 1000 mL
steadylite plus Reporter Gene Assay System, 1000 mL
Cayman Chemical Kaempferol 3-O-galactosIde 1mg
A flavonoid with diverse biological activities; inhibits sPLA2 (IC50 = 17.6 µM); decreases protein levels of Akt and induces apoptosis in NCI H460 NSCLC cells at 12.5, 25, and 50 µM; scavenges DPPH radicals and superoxide anions in cell-free assays at 200 UG/ml; reduces TPA-induced EAr edema and carrageenan-induced paw edema in mice at 150 mg/kg
Creative Biomart Active Recombinant Firefly Luciferase Protein (1-550), N-His-tagged, 1mg
Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP, produces green light with a wavelength of 562 nm. Luciferase from firefly is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening.
Cayman Chemical Kaempferol 3-O-galactosIde 5mg
A flavonoid with diverse biological activities; inhibits sPLA2 (IC50 = 17.6 µM); decreases protein levels of Akt and induces apoptosis in NCI H460 NSCLC cells at 12.5, 25, and 50 µM; scavenges DPPH radicals and superoxide anions in cell-free assays at 200 UG/ml; reduces TPA-induced EAr edema and carrageenan-induced paw edema in mice at 150 mg/kg