General Cell Based Assays
Eurofins Discoverx PathHunter eXpress CMKLR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0313E2CP0M)
The PathHunter CMKLR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. chemerin) based activation of CMKLR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CMKLR1 and EA-tagged β-Arrestin-2. Activation of CMKLR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR37 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0339E2ACP1M)
The PathHunter GPR37 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR37 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR37 and EA-tagged β-Arrestin-2. Activation of GPR37 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human CHRM4 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human CHRM4 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure CHRM4 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human XBP1 Nuclear Translocation Cell-Based Assay Kit (U2OS)
The PathHunter Human XBP1 Stable Cell Line Nuclear Translocation Assay (U2OS) contains a stable clonal cell line used to measure the Endoplasmic Reticulum stress response via nuclear translocation of XBP1. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Discovery Life Sciences Gentest Monkey Hep Rhesu
Gentest Monkey Hepatocytes Rhesus Cryopreserved Plateable Inducible
Cayman Chemical HemoglobIn ColorImetrIc asy
Hemoglobin (Hb or Hgb) is a globular protein found primarily in erythrocytes that carries oxygen from the lungs to tissue where it releases oxygen and then returns carbon dioxide (CO2) from the tissue to the lungs. Hemoglobin also binds CO2, carbon monoxide (CO), and nitric oxide (NO). Cayman’s Hemoglobin Colorimetric Assay provides a quick, reliable method for determining total hemoglobin concentration in a variety of biological samples, including blood, tissue homogenates and cell lysates. Unlike the universally accepted reference method of hemoglobin determination, which uses potassium cyanide as a reagent and commonly under-estimates hemoglobin levels, Cayman’s Hemoglobin Colorimetric Assay utilizes an optimized detection reagent which is non-toxic, reporting accurate measurements of total hemoglobin concentrations with an absorbance between 560-590 nm.
Dojindo Molecular Technologies Inc NAD/NADH Assay Kit-WST (100TESTS), enables quantification of the amount of total NAD+/NADH, NAD+ and NADH in cells and measurement of their ratio.
Recently, it has become clear that Sirtuin is linked to longevity and plays a role in NAD+ level regulation. Also Sirtuin has been recognized as a marker necessary to understanding biological states, such as obesity & diabetes, as well as cellular differentiation. Cell lysate from a cell culture can be easily prepared via Deproteinzation using the extraction buffer and filtration tubes found within this kit. Intracellular NADH levels can be quantified by heat treatment of cell lysate. Additionally, intracellular NAD+ levels can be determined by subtracting NADH levels from independently measured total NAD+/NADH levels. NAD/NADH Assay Kit-WST enables quantification of the amount of total NAD+/NADH, NADH and NAD+ in cells and measurement of their ratio. Dojindo has various quantification kits of Intracellular Metabolites. (https://www.dojindo.com/quantification-of-intracellular-metabolites/)
Cayman Chemical LDL Uptake CellBased Assay Ki
LDL uptake and its regulation are important therapeutic targets for atherosclerosis and related diseases. Cayman Chemical’s LDL Uptake Cell-Based Assay employs a preparation of human LDL conjugated to Dylight™ 550 as a fluorescent probe for detection of LDL uptake into cultured cells. A LDL receptor-specific antibody and a Dylight™ 488-conjugated secondary antibody are included in the kit for identifying the distribution of LDL receptors. The kit provides a convenient tool for studying LDL uptake and its regulation at the cellular level.
MOLECULAR DEPOT LLC Pyruvate Kinase Rabbit - 10 kU
Pyruvate Kinase from Rabbit Muscle is native Pyruvate Kinase purified from the rabbit muscle This product has been used as molecular tool for various biochemical applications It has also been used in a wide array of other chemical and immunological applications For in vitro research use only
Eurofins Discoverx cAMP Hunter™ Human AVPR2 Gs Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human AVPR2 Gs Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure AVPR2 (GPCR) activity via increased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.