General Cell Based Assays
Cayman Chemical Acid Phosphatase Colorimetric
Cayman’s Acid Phosphatase Assay provides a convenient method for detecting AP activity in plasma, serum, urine, and semen. The assay utilizes para-nitrophenyl phosphate (pNPP) as a chromogenic substrate for the enzyme. In the first step, AP dephosphorylates pNPP. In the second step, the phenolic OH-group is deprotonated under alkaline conditions resulting in p-nitrophenolate that yields an intense yellow color which can be measured at 405-414 nm. The kit provides all reagents needed to assay AP activity, and includes L-tartrate, an inhibitor of non-tartrate resistant APs.
Eurofins Discoverx PathHunter Human BMP-6 (BMPR1B/ACVR2B) Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human BMP-6 (BMPR1B/ACVR2B) Stable Cell Line Dimerization Assay (U2OS) measures ligand-induced dimerization of BMPR1B/ACVR2B. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human PTGER3 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human PTGER3 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure PTGER3 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Meso Scale Discovery U-PLEX Human TNF-B ASY 25 PL
The Meso Scale Discovery U-PLEX Human TNF-beta Assay Kit is a multiplex immunoassay that measures human TNF-beta in serum plasma cell culture supernatants and other biological samples It utilizes electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and requires low sample volume The assay can be run on Meso Scale Discovery instrumentation including the QuickPlex SQ 120 QuickPlex SQ 120i MESO QuickPlex SQ 120 and V-PLEX Plus The kit includes a 96-well plate coated with capture antibodies detection antibodies calibrators and ancillary reagents needed to perform the assay
Eurofins Discoverx PathHunter eXpress GPR142 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0413E2ACP1L)
The PathHunter GPR142 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR142 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR142 and EA-tagged β-Arrestin-2. Activation of GPR142 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cellular Dynamics International iCell Microglia Media Kit, 50 ml iCell Glial Base Medium, 0.5 ml iCell Microglia Supplement A (100x), 0.5 ml iCell Microglia Supplement B (100x), 1 ml iCell Neural Supplement C (50X)
The iCell Microglia Media Kit is a specialized cell culture medium tailored for the optimal growth and maintenance of human iPSC-derived microglia. It provides a consistent in vitro environment, essential for research in microglial biology and neurodegenerative diseases. This media kit solves the challenges faced with primary human microglia by ensuring reproducible results for various experiments related to neuroinflammation and drug discovery.
- Supports growth of iPSC-derived human microglia.
- Includes multiple supplements for enhanced performance.
- Stores complete medium at 4°C for up to 2 weeks.
- Can be aliquoted for single-use storage at -20°C for up to 3 months.
- Recommended culture conditions: 37°C and 5% CO2.
- Designed for research purposes only, focusing on neurodegeneration and drug discovery.
- Maintains relevant microglial markers and physiological functions.
Eurofins Discoverx PathHunter Human BDKRB2 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human BDKRB2 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure BDKRB2 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx SPRINTer™ Human BRD4 Targeted Protein Degradation Cell-Based Assay Kit (HCT-116)
The SPRINTer™ Human BRD4 Targeted Protein Degradation Stable Cell Line Assay (HCT-116) contains a stable clonal cell line used for monitoring drug-induced protein turnover of BRD4, supporting TPD studies. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human GPR139 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR139 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR139 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human GPR152 β-Arrestin Cell-Based Assay Kit (HEK 293)
The PathHunter Human GPR152 β-Arrestin Stable Cell Line Assay (HEK 293) contains a stable clonal cell line used to measure GPR152 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress NTSR1 Activated GPCR Internalization Assay, 2pl Kit. (93-0671E3CP5M)
The PathHunter NTSR1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of NTSR1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged NTSR1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting NTSR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CXCR7 Activated GPCR Internalization Assay, 10pl Kit. (93-0738E3CP27L)
The PathHunter CXCR7 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CXCR7 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CXCR7. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CXCR7-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PTHR2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0306E2CP0M)
The PathHunter PTHR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. PTH) based activation of PTHR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTHR2 and EA-tagged β-Arrestin-2. Activation of PTHR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Innovative Research Inc Rat Act tPA Kit
Rat Active tPA ELISA Kit - This rat tPA activity assay is intended for the quantitative determination of active tissue plasminogen activator (tPA) in rat plasma and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. Functionally active rat tPA will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Only free active tPA will react with the PAI-1 bound to the plate. After appropriate washing steps, polyclonal anti-tPA primary antibody binds to the captured tPA. Excess antibody is washed away and bound primary antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate is used for color development at 450nm. The amount of color development is directly proportional to the concentration of active tPA in the sample.
Eurofins Discoverx PathHunter eXpress GPR149 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0420E2ACP1M)
The PathHunter GPR149 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR149 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR149 and EA-tagged β-Arrestin-2. Activation of GPR149 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.