General Cell Based Assays
Eurofins Discoverx cAMP Hunter eXpress CRHR2 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0048E2CP2L)
The cAMP Hunter CRHR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CRH, sauvagine) based activation of CRHR2 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CRHR2, a naturally Gs coupled GPCR. Upon activation of CRHR2, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress GALR1 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0058E2CP2M)
The cAMP Hunter GALR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. galanin) based activation of GALR1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GALR1, a naturally Gi/Go coupled GPCR. Upon activation of GALR1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical Acetaldehyde asy Reagent 200uL
A Clk1 Clk2 and Clk4 inhibitor (IC50s 8 20 and 12 nM respectively) selective for these kinases over Clk3 (IC50 >3000 nM) as well as DYRK1A DYRK1B DYRK2 HIPK1 and HIPK3 (IC50s 213 956 1309 187 and 159 nM respectively) but does inhibit HIPK2 (IC50 23 nM)
Cayman Chemical Cel-Based asy nxIn V BI 50mL
A TRPV4 and TRPA1 inhibitor (IC50s 4.19 and 5.56 respectively for the rat channels) inhibits GSK1016790A-induced calcium influx in whole-cell patch-clamp assays using Neuro2a cells expressing TRPV4 at 5 UM prevents compression-induced increases in NF-KB transcriptional activity and secreted levels of IL-6 and VEGFA as well as inhibits compression-induced fractures abnormal cell morphology and fissures in the intervertebral space in primary mouse lumbar spines at 10 UM
Eurofins Discoverx cAMP Hunter eXpress CXCR1 (IL8RA) CHO-K1 GPCR Assay, 2-Plate Kit. (95-0073E2CP2M)
The cAMP Hunter CXCR1 (IL8RA) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL8/IL8) based activation of CXCR1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CXCR1, a naturally Gi/Go coupled GPCR. Upon activation of CXCR1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Montana Molecular Human Somatostatin Receptor Type 2 (SSTR2) Big Sky BacMam, 15mL
Human Somatostatin Receptor Type 2 packaged in Big Sky BacMam for mammalian cell expression. BacMam packaging provides efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in primary or iPSC-derived cells. BacMam expression is easily titrateable to suit your experimental needs.
Eurofins Discoverx cAMP Hunter eXpress CHRM2 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0078E2CP2M)
The cAMP Hunter CHRM2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. oxotremorine) based activation of CHRM2 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CHRM2, a naturally Gi/Go coupled GPCR. Upon activation of CHRM2, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress DRD1 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0083E2CP2L)
The cAMP Hunter DRD1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine) based activation of DRD1 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type DRD1, a naturally Gs coupled GPCR. Upon activation of DRD1, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress CHRM2 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0078E2CP2L)
The cAMP Hunter CHRM2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. oxotremorine) based activation of CHRM2 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CHRM2, a naturally Gi/Go coupled GPCR. Upon activation of CHRM2, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter NPY2R Bioassay Kit, 10-Plate. (95-0077Y2-00056)
The cAMP Hunter NPY2R Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. peptide YY) based activation of NPY2R GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type NPY2R, a naturally Gi coupled GPCR. Upon activation of NPY2R, the (decrease/increase) in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx cAMP Hunter eXpress CXCR4 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0081E2CP2L)
The cAMP Hunter CXCR4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL12/SDF-1α) based activation of CXCR4 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CXCR4, a naturally Gi/Go coupled GPCR. Upon activation of CXCR4, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress C5AR1 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0079E2CP2L)
The cAMP Hunter C5AR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. C5a) based activation of C5AR1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type C5AR1, a naturally Gi/Go coupled GPCR. Upon activation of C5AR1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress GHRHR CHO-K1 GPCR Assay, 2-Plate Kit. (95-0076E2CP2M)
The cAMP Hunter GHRHR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. GRF) based activation of GHRHR GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GHRHR, a naturally Gs coupled GPCR. Upon activation of GHRHR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter Exenatide Bioassay Kit, 10-Plate. (95-0062Y2-00102)
The cAMP Hunter Exenatide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Exenatide based activation of GLP1R GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GLP1R, a naturally Gs coupled GPCR. Upon activation of GLP1R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.