General Cell Based Assays
Eurofins Discoverx PathHunter eXpress CMKLR1 Activated GPCR Internalization Assay, 10pl Kit. (93-0720E3CP22L)
The PathHunter CMKLR1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CMKLR1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CMKLR1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CMKLR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CMKLR1 Activated GPCR Internalization Assay, 2pl Kit. (93-0720E3CP22M)
The PathHunter CMKLR1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CMKLR1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CMKLR1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CMKLR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL4R/IL13RA1 Dimerization Assay, HEK293,10pl Kit. (93-1001E1CP0L)
The PathHunter IL4R/IL13RA1 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-13) induced dimerization of IL4R/IL13RA1 receptors. This product uses EFC technology. The cell line included overexpresses IL4R target protein fused to PK alongside IL13RA1 target protein fused to EA. Upon ligand-induced dimerization of IL4R/IL13RA1, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
BioVendor, LLC QuickZyme Sensitive Tissue Collagen Assay Kit 5 X 96-Assays
QuickZyme Sensitive Tissue Collagen Assay Kit, 5 X 96-Assays. Measure hydroxyproline in small tissue samples with low collagen levels! The ONLY hydroxyproline-based assay which shows no matrix effect and no need for further dilution of the hydrolysate, enabling measurement in small samples with low hydroxyproline levels, such as liver. No drying of hydrolysate is needed, resulting in a fast (<2 hrs), sensitive and easy (96-well plate format) assay. Dynamic range: 6-300 ug/ml. Sensitivity: 2.5 ug/ml. Sample vol. 50-500 ul. Species independent. Measures total hydroxyproline. If this hydroxyproline is obtained upon hydrolysis of collagen, it represents all collagen types present without discriminating between types of collagen, procollagen, mature collagen and collagen degradation products. Hydroxyproline in tissue hydrolysates can be used as a direct measure of the amount of collagen present. Collagen hydrolysis tubes & protocol ARE included. Ease-of-use: Equivalent to ELISA.
Abcam Comet Assay Kit (3 well slides)
Comet Assay Kit (3 well slides)
The product is subject to the following: Abcam Restricted Use Statement
Cell Signaling Technology TUNEL Assay Kit Fluorescence
TUNEL Assay Kit (Fluorescence, 640 nm) 1 Kit;
Indigo Biosciences Human Adrenoceptor Beta 1 (ADRB1) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Adrenoceptor Beta 1 reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against ADRB1. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Meso Scale Discovery U-PLEX Human Nectin-4 ASY
The Meso Scale Discovery Human Nectin-4 Singleplex Assay Kit is a singleplex immunoassay that measures Nectin-4 levels in human serum plasma and cell culture supernatants This assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity wide dynamic range and low sample volume requirements The assay requires 50 L of sample and can be read on Meso Scale Discovery SECTOR Imagers The assay has a typical lower limit of detection of 0 03 ng/mL and an upper limit of quantification of 2500 ng/mL This assay kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer calibrator and control
Eurofins Discoverx PathHunter Human F2RL3 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human F2RL3 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure F2RL3 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human AVPR2 β-Arrestin Cell-Based Assay Kit (HEK 293)
The PathHunter Human AVPR2 β-Arrestin Stable Cell Line Assay (HEK 293) contains a stable clonal cell line used to measure AVPR2 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Abcam Monoamine Oxidase (MAO) Assay Kit
Monoamine Oxidase (MAO) Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
ILEX LIFE SCIENCES LLC QuickZyme Human MMP-7 Activity Assay Kit, 96 Assays, 96-well plate format, immunoassay for active or total matrix metalloproteinase 7 (conditioned culture medium, serum, urine, tissue homogenates samples)
The QuickZyme Human MMP-7 Activity Assay is an enzyme activity immunoassay that enables you to specifically measure both endogenous active MMP-7 and total active MMP-7 (following activation of pro-MMP-7 on the plate with APMA). It can be used for the measurement of MMP-7 activity in various biological samples, such as conditioned culture media, tissue homogenates, serum, plasma and urine. This 96-well plate format assay is based on the QuickZyme technology, using a modified pro-enzyme as a substrate, which upon activation is able to release color from a chromogenic peptide substrate. This multiplication step provides a unique assay sensitivity. ASSAY RANGE: 0.004 ¿ 16 ng/ml. SENSITIVITY: 30 pg/ml (3 h incubation); 4 pg/ml (25 h incubation). SYNONYMS: Matrix metalloproteinase-7, MMP7, Matrilysin, Matrilysin-1, Matrin, Pump-1 protease, Uterine metalloproteinase.
Meso Scale Discovery R-PLEX Rat β-NGF Assay, 5 plate size, Singleplex format, 96-well plate, Plasma and Serum sample types, Dynamic range 0.2 - 6000 pg/mL, LLOD 0.2 pg/mL, For research use only
R-PLEX Rat β-NGF Assay, 5 plate size, Singleplex format, 96-well plate, Plasma and Serum sample types, Dynamic range 0.2 - 6000 pg/mL, LLOD 0.2 pg/mL, For research use only
Indigo Biosciences INDIGO Biosciences Human GIPR Reporter Assay System, 384-well Format, Luciferase
This Human GIPR Reporter Assay System is a cell-based luciferase reporter assay kit for quantifying functional activity against the Human Gastric Inhibitory Polypeptide Receptor (GIPR). It uses proprietary mammalian cells and an engineered luciferase reporter gene, delivering clear, reproducible results for 384-well format compound screening.
- Utilizes proprietary human cells with constitutive GIPR expression.
- Engineered luciferase reporter gene functionally linked to Cyclic AMP Response Elements (CRE).
- All-inclusive system with cells, media, reference activator, and detection reagents.
- Provides low background, high sensitivity, and broad linear dynamic range via bio-luminescence.
- Ensures exceptional cell viability post-thaw and consistent lot-to-lot results.
Indigo Biosciences INDIGO BIOSCIENCES INC Assay, FGFR1c/α-Klotho, Endocrine FGF Signaling, 1x 384-well
This product is an assay for Endocrine FGF Signaling.
- Uses proprietary human cells engineered to provide constitutive expression of Human Fibroblast Growth Factor Receptor 1, isoform 1 and Human Fibroblast Growth Factor Receptor 2, isoform 1.
- Incorporates a luciferase reporter gene functionally linked to tandem NFAT consensus response element sequences.
- Provides an all-inclusive cell-based reporter assay system.
- Includes Reporter Cells, optimized culture medium, heparin-supplemented culture medium, FGF-Acidic (physiological paracrine activator of FGFR1/2), Luciferase Detection Reagents, and a cell culture-ready assay plate.
- Kit materials are sufficient to perform assays in a single 384-well assay plate.
- Cryopreserved mammalian cells are provided and require storage at -80°C until rapid-thaw.
- Intended for research purposes only.