General Cell Based Assays
Revvity Health Sciences Inc HTRF CD16a (FcgRIIIa, F158) Cellular Binding Kit, 500 Assay Points
HTRF CD16a (FcgRIIIa, F158) Cellular Binding Kit, 500 Assay Points
Abcam LDH Assay Kit¿(Cytotoxicity)
LDH Assay Kit(Cytotoxicity)
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical AMPK actIvatr 5mg
An indirect activator of AMPK (EC50 = 11.7 μM); stimulates glucose uptake in L6 myocytes and promotes phosphorylation of ACC, a known target of AMPK; decreases blood glucose concentrations in Zucker diabetic rats, demonstrating its utility in whole animals; inhibits the growth of multiple myeloma cells
Echelon Biosciences Research Labs PI(3,4,5)P3 DiC16 1 mg
PI(3,4,5)P3 diC16 (Phosphatidylinositol 3,4,5-trisphosphate diC16) is a synthetic, purified dipalmitoyl PI(3,4,5)P3.Phosphoinositides (PIPns) are minor components of cellular membranes but are integral signaling molecules for cellular communication. Phosphatidylinositol 3,4,5-trisphosphate (PIP3), formed from PI(4,5)P2 though phosphorylation by PI 3-kinase, activates numerous signaling pathways resulting in cell proliferation, growth, survival, glucose transport and protein synthesis. High PIP3 levels from disregulation of PI3-K have been demonstrated in cancer and inflammatory diseases. PIP3 is hydrolyzed by the phosphatases PTEN to PI(4,5)P2 and SHIP to PI(3,4)P2. This item is non returnable
Eurofins Discoverx PathHunter eXpress FGFR4 Functional Assay, 2-Plate Kit. (93-1022E3CP16M)
The PathHunter eXpress FGFR4 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated FGFR4 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged FGFR4 and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Indigo Biosciences Human Adrenoceptor Beta 1 (ADRB1) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Adrenoceptor Beta 1 reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against ADRB1. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Revvity Health Sciences Inc HTRF Phospho-IR-β (Insulin Receptor Beta) (Tyr1150 /1151) Detection Kit, 50,000 Assay Points
HTRF Phospho-IR-β (Insulin Receptor Beta) (Tyr1150 /1151) Detection Kit, 50,000 Assay Points
Eurofins Discoverx InCELL Hunter eXpress PIM1 Kinase Binding Assay, 2pl Kit. (96-0052E15CP0M)
The InCELL Hunter eXpress PIM1 Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of PIM1 via detection of PIM1 levels. This product uses EFC technology. The cell line included overexpresses PIM1 target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress ADRB3 DLD1 GPCR Assay, 2-Plate Kit. (95-0182E13CP25M)
The cAMP Hunter ADRB3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. isoproterenol) based activation of ADRB3 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADRB3, a naturally Gs coupled GPCR. Upon activation of ADRB3, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress FPR3 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0171E2CP2M)
The cAMP Hunter FPR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. humanin) based activation of FPR3 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type FPR3, a naturally Gi/Go coupled GPCR. Upon activation of FPR3, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR35 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0942E2CP2M)
The PathHunter GPR35 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. zaprinast) based activation of GPR35 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR35 and EA-tagged β-Arrestin-2. Activation of GPR35 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human MMP-3 ASY
The R-PLEX Human MMP-3 Assay Kit from Meso Scale Discovery is used to quantitatively measure MMP-3 levels in human serum plasma saliva urine cell culture supernatant and other biological samples It utilizes electrochemiluminescence (ECL) detection technology and provides high sensitivity a broad dynamic range and requires low sample volume The assay has high specificity for active MMP-3 with no cross-reactivity to pro-MMP-3 The kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer diluent and calibrator It can detect MMP-3 levels between 0 03-1000 ng/mL
Abcam UGT Activity Assay / Ligand Screening Kit (Fluorometric)
UGT Activity Assay / Ligand Screening Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
BioVendor, LLC Sircol; INSOLUBLE Collagen Assay, Bovine Collagen (Standard Kit Size: 100 assays)
Sircol; INSOLUBLE Collagen Assay, Bovine Collagen (Standard Kit Size: 100 assays). The Sircol™ INSOLUBLE Collagen Assay is a dye-binding method for analysis of insoluble collagen fibers. The assay be used to assess the rate of production of newly laid down collagen fibers during periods of rapid growth, development, tissue repair, remodeling and wound healing. Sources of material includes tissues, bone and calcified tissue. It replaces the conventional hydroxyproline analysis. No more working with concentrated acids or alkalis! The Sircol Insoluble Assay uses mild acid and temperature treatment for 2 to 3 hrs. The solubility conversion to denatured collagen and subsequent measurement can be completed within one working day. The ratio of soluble and insoluble collagens can be measured using the same Sircol Dye Reagent. Multispecies colorimetric assay. Detection Limit: 10.0 g. Assay-time: 4 hours. Biocolor Item # S2000
Eurofins Discoverx cAMP Hunter eXpress FPRL1 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0044E2CP2L)
The cAMP Hunter FPRL1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of FPRL1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type FPRL1, a naturally Gi/Go coupled GPCR. Upon activation of FPRL1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.