General Cell Based Assays
Enzo Life Sciences Blebbistatin(+/-), (5mg), CAS Number: 674289-55-5
Inhibitor of non-muscle myosin II. Blebbistatin (so named because of its ability to block cell blebbing) is a selective and high-affinity (IC50 approx. 4 µM) inhibitor of non-muscle myosin II. During cell division blebbistatin inhibits contraction of the cleavage furrow without disrupting mitosis. It inhibits pancreatic adenocarcinoma cellular invasiveness and PDGF-stimulated fibroblast-collagen matrix contraction. Formula: C18H16N2O2. MW: 292.3. CAS Number: 674289-55-5. Purity: ≥97%. Appearance: Bright yellow solid. Solubility: Soluble in DMSO (25mg/ml). Long Term Storage: -20°C
Bioassay Systems EnzyFluo™ AMPK Phosphorylation Assay Kit. For quantitative fluorescent immunoenzymatic assay of AMPK phosphorylation status in cultured cells.
For quantitative fluorescent immunoenzymatic assay of AMPK phosphorylation status in cultured cells. Key Features: Sensitive. Can measure pAMPK modulation in as little as 500 cells/well. New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL360/450nm, 530/585nm. Samples: Cells. Species: Human, mouse, rat. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.
Meso Scale Discovery R-PLEX Human IL1RL1/ST2 ASY
The R-PLEX Human IL1RL1/ST2 Assay Kit from Meso Scale Discovery is a multiplex immunoassay that measures IL1RL1/ST2 in human serum plasma and cell culture supernatants This assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5-logs of dynamic range and low sample volume requirements The kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with an ECL compound and calibrators and controls to generate a standard curve The assay takes 3 5 hours to complete and requires a Meso Scale Discovery instrument such as SECTOR Imager QuickPlex SQ 120 or QuickPlex SQ 120
American Research Products Inc Canine acetylcholine receptor
Canine acetylcholine receptor antibody AChRab ELISA Kit from CUSABIO Detection Range 3 75 ng/mL-240 ng/mL Sensitivity 0 94 ng/mL Sample serum plasma Method Sandwich
Eurofins Discoverx PathHunter eXpress GPR26 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0392E2ACP1L)
The PathHunter GPR26 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR26 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR26 and EA-tagged β-Arrestin-2. Activation of GPR26 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CHRM5 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0519E2CP0L)
The PathHunter CHRM5 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. oxotremorine) based activation of CHRM5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CHRM5 and EA-tagged β-Arrestin-2. Activation of CHRM5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Biochain Institute Inc ATP Assay Kit, 100; assays/PK
BioChain's EnzyLightTM ATP Assay Kit provides a rapid method to measure intracellular ATP. The single working reagent lyses cells to release ATP, which, in the presence of luciferase, immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATPconcentration. This non-radioactive, homogeneous cell-based assay is performed in microplates. The reagent is compatible with all culture media and with all liquid handling systems for high throughput screning.
Biochain Institute Inc NAD/NADH Assay Kit, 100 Assays/PK
BioChain's NAD+/NADH assay kit provides simple, direct and automation-ready procedure to measure NAD+/NADH concentration. It based on an alcohol dehydrogenase cycling reaction, in which a tetrazolium dye (MTT) is reduced by NADH in the presence of phenazine methosulfate (PMS). The intensity of the product color, measured at 565 nm, is proportionate to the NAD+/NADH concentration in the sample.
Eurofins Discoverx PathHunter eXpress FPR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0314E2CP0M)
The PathHunter FPR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. formylpeptides) based activation of FPR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged FPR1 and EA-tagged β-Arrestin-2. Activation of FPR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.