General Cell Based Assays
Eurofins Discoverx PathHunter eXpress EDG1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0207E2CP4L)
The PathHunter EDG1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. S1P) based activation of EDG1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged EDG1 and EA-tagged β-Arrestin-2. Activation of EDG1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TGFBR1/ACVR2 Dimerization Assay, 2pl Kit. (93-1012E3CP5M)
The PathHunter TGFBR1/ACVR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. GDF-11) induced dimerization of TGFBR1/ACVR2 receptors. This product uses EFC technology. The cell line included overexpresses TGFBR1 target protein fused to PK alongside ACVR2 target protein fused to EA. Upon ligand-induced dimerization of TGFBR1/ACVR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL7R/CRLF2 Dimerization Assay, 10pl Kit. (93-1019E13CP25L)
The PathHunter IL7R/CRLF2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. TSLP) induced dimerization of IL7R/CRLF2 receptors. This product uses EFC technology. The cell line included overexpresses IL7R target protein fused to PK alongside CRLF2 target protein fused to EA. Upon ligand-induced dimerization of IL7R/CRLF2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress RXFP3 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0692E2CP2L)
The PathHunter RXFP3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. relaxin-3) based activation of RXFP3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged RXFP3 and EA-tagged β-Arrestin-2. Activation of RXFP3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam HDAC substrate, colorimetric (Boc-Lys(Ac)-pNA), 25MG
MW 408.45 g/mol, Purity >98%. Colorimetric substrate for histone deacetylases (HDAC). For use in HDAC activity assays.
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress OPRM1 U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0213E3CP0M)
The PathHunter OPRM1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. met 5-enkephalin) based activation of OPRM1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OPRM1 and EA-tagged β-Arrestin-2. Activation of OPRM1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
TRIBIOSCIENCE INC Cluster of Differentiation 38 (CD38 Cyclase) Activity Assay Kit (Fluorometric), 100 Tests
CD38 Assay Kit is a simple and fast fluorometric assay that measures the cyclase activity of CD38 in biological samples. CD38 catalyzes the conversion of nicotinamide guanine dinucleotide (NGD+) to cyclic GDP-ribose (cGDPR) which can be measured by fluorometric method at Ex/Em=300 nm/410 nm.
Abcam HDAC substrate, colorimetric (Boc-Lys(Ac)-pNA), 100MG
MW 408.45 g/mol, Purity >98%. Colorimetric substrate for histone deacetylases (HDAC). For use in HDAC activity assays.
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress VIPR2 Total Internalization Assay, 10pl Kit. (93-0824E3CP5L)
The PathHunter VIPR2 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of VIPR2 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged VIPR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the VIPR2 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress NPSR1b U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0822E3CP0L)
The PathHunter NPSR1b eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. neuropeptide S) based activation of NPSR1b GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged NPSR1b and EA-tagged β-Arrestin-2. Activation of NPSR1b stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PTGIR Total Internalization Assay, 2pl Kit. (93-0838E3CP0M)
The PathHunter PTGIR Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of PTGIR via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged PTGIR and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the PTGIR receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress KDR/KDR Dimerization Assay, 2-Plate Kit. (93-0996E1CP0M)
The PathHunter KDR/KDR Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. VEGF-121) induced dimerization of KDR/KDR receptors. This product uses EFC technology. The cell line included overexpresses KDR target protein fused to PK alongside KDR target protein fused to EA. Upon ligand-induced dimerization of KDR/KDR, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.