General Cell Based Assays
Eurofins Discoverx PathHunter IL17RA/IL17RC Dimerization Bioassay Kit, 10pl. (93-0999Y3-00054)
The PathHunter IL17RA/IL17RC Dimerization Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. IL-17) induced dimerization of IL17RA/IL17RC receptors. This product uses EFC technology. The cell line included overexpresses IL17RC target protein fused to PK alongside IL17RA target protein fused to EA. Upon ligand-induced dimerization of IL17RA/IL17RC, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress rCHRM4 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0870E2RCP2M)
The PathHunter rCHRM4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. acetylcholine) based activation of rCHRM4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rCHRM4 and EA-tagged β-Arrestin-2. Activation of rCHRM4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress cINSRa Functional Assay, 10-Plate Kit. (93-1016E3CP16L)
The PathHunter eXpress cINSRa Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated cINSRa dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged cINSRa and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Cynomolgus monkey version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Human MCP-1 ASY 25 PL
The Meso Scale Discovery U-PLEX Human MCP-1 Assay Kit quantifies monocyte chemoattractant protein 1 (MCP-1) in human serum plasma and cell culture supernatants Using MSD s SULFO-TAG labels and multi-array technology it measures MCP-1 levels down to 0 4 pg/mL The kit includes a 96-well plate with capture antibodies detection antibodies buffers blockers calibrators and controls The assay takes about 3 5 hours and requires an MSD instrument like the MESO QuickPlex SQ 120 It offers high sensitivity broad range and minimal sample volume for efficient MCP-1 analysis
Cayman Chemical Glycogen FluorometrIc Detecto
A GnRH receptor antagonist (Kis 6 2.2 and 3.8 nM for the human mouse and rat receptors respectively) selective for the GnRH receptor over a panel of 43 receptors ion channels and enzymes at 1 UM prevents GnRH-induced increases in inositol phosphate production in HEK293 cells expressing the human GnRH receptor (KB 25 nM) oral or intravenous administration decreases plasma LH levels in castrated rats inhibits GnRH-A-induced increases in plasma LH and testosterone levels in non-castrated rats at 20 mg/kg
Cayman Chemical cGAS Stop SolutIon 1mL
cGAS Stop Solution has been tested and formulated to work exclusively with Cayman’s cGAS Inhibitor Screening Assay Kit (Item No. 701930).
Bioassay Systems EnzyChrom™ Adipolysis Assay Kit. For quantitative assay of adipolysis and evaluation of drug effects on adipolysis.
For quantitative assay of adipolysis and evaluation of drug effects on adipolysis. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 0.92 to 100 µg/mL (10 to 1000 µM) glycerol for colorimetric assays and 0.2 to 5 µg/mL for fluorimetric assays. Rapid and convenient.The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Robust and amenable to HTS assays. Potential interference by testing drugs is greatly reduced at 570nm. Compatible with culture media containing phenol red. Assays can be performed in 96 or 384-well plates. Method: OD570nm, or FL530/585nm. Samples: Cell culture media. Species: All. Procedure: Assay takes 20 min. Kit size: 200 tests. Detection limit: 0.92 µg/mL.
Epigentek Inc Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Fluorometric) (96 assays)
The Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Fluorometric) is a complete set of optimized buffers and reagents for measuring activity/inhibition of total cytosine oxygenase TET enzymes in nuclear extracts or purified TET isoforms (TETs 1-3) from a broad range of species such as mammals, plants, fungi, and bacteria, and in a variety of forms including, but not limited to cultured cells and fresh and frozen tissues.
Eurofins Discoverx PathHunter eXpress mOPRK1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0480E2MCP0M)
The PathHunter mOPRK1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dynorphin A) based activation of mOPRK1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mOPRK1 and EA-tagged β-Arrestin-2. Activation of mOPRK1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress SCTR CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0237E2CP0L)
The PathHunter SCTR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. secretin) based activation of SCTR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged SCTR and EA-tagged β-Arrestin-2. Activation of SCTR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Human IFN-α Detection Kit, 500 Assay Points
HTRF Human IFN-α Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Pro-Collagen Type 1 Detection Kit Standard
HTRF Human Pro-Collagen Type 1 Detection Kit Standard
Eurofins Discoverx PathHunter eXpress P2RY6 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0500E2CP19L)
The PathHunter P2RY6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. UTP) based activation of P2RY6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged P2RY6 and EA-tagged β-Arrestin-2. Activation of P2RY6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Rat β-NGF Assay, 5 plate size, Singleplex format, 96-well plate, Plasma and Serum sample types, Dynamic range 0.2 - 6000 pg/mL, LLOD 0.2 pg/mL, For research use only
R-PLEX Rat β-NGF Assay, 5 plate size, Singleplex format, 96-well plate, Plasma and Serum sample types, Dynamic range 0.2 - 6000 pg/mL, LLOD 0.2 pg/mL, For research use only