General Cell Based Assays
Eurofins Discoverx PathHunter eXpress PRLR-JAK1 Functional Assay, 10-Plate Kit. (93-0686E3CP0L)
The PathHunter PRLR-JAK1 Functional assay eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting CTKs. This kit assesses PRLR activation via detection of JAK1 mediated phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged PRLR cytokine receptor, an untagged CTK JAK1 and EA-tagged SH2 domain protein. Upon activation of the cytokine receptor, CTK-based receptor phosphorylation leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CDC25A Degradation Assay, 2-Plate Kit. (93-0765E3CP0M)
The PathHunter eXpress CDC25A Degradation Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting the cell cycle checkpoint pathway. This kit enables detection of levels of CDC25a, allowing for assessment of CHK-1-activation-induced CDC25a degradation. This product uses EFC technology. The cell line included overexpresses CDC25a target protein fused to ProLabel. EA is contained in the detection reagent. Upon pathway-activation-induced degradation of CDC25a EFC signal is lost. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Growth Hormone Bioassay Kit, 2pl. (93-0756Y3-00023)
The PathHunter Growth Hormone Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses human growth hormone-induced activation of GHR cytokine receptors via detection of SH2 domain protein recruitment. The included cell line overexpresses ProLink-tagged GHR target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress DRD4 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0690E2CP0L)
The PathHunter DRD4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine) based activation of DRD4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged DRD4 and EA-tagged β-Arrestin-2. Activation of DRD4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PTHR1 Activated GPCR Internalization Assay, 10pl Kit. (93-0770E3CP0L)
The PathHunter PTHR1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of PTHR1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged PTHR1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting PTHR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CHRM3 U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0860E3CP0L)
The PathHunter CHRM3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. acetylcholine) based activation of CHRM3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CHRM3 and EA-tagged β-Arrestin-2. Activation of CHRM3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress c-KIT Functional Assay, 10-Plate Kit. (93-0853E3CP16L)
The PathHunter eXpress c-KIT Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated c-KIT dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged c-KIT and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the cynomolgus version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress EphB2 Functional Assay, 10-Plate Kit. (93-0848E3CP16L)
The PathHunter eXpress EphB2 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated EphB2 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged EphB2 and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TAAR5 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0718E2CP1M)
The PathHunter TAAR5 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of TAAR5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged TAAR5 and EA-tagged β-Arrestin-2. Activation of TAAR5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress OXTR Activated GPCR Internalization Assay, 2-Plate Kit. (93-0715E3CP0M)
The PathHunter OXTR Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of OXTR via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged OXTR. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting OXTR-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress RXFP3 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0692E2CP2L)
The PathHunter RXFP3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. relaxin-3) based activation of RXFP3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged RXFP3 and EA-tagged β-Arrestin-2. Activation of RXFP3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress XBP1 Nuclear Translocation Assay, 10pl Kit. (93-0857E3CP5L)
The PathHunter eXpress XBP1 Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the IRE1/XBP1 pathway. This kit assesses ligand (e.g. thapsigargin) based pathway activation via detection of resulting XBP1 nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged XBP1 and nuclear localized EA. Upon pathway activation, induced nuclear translocation of XBP1 leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress NPBWR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0818E2CP2M)
The PathHunter NPBWR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. neuropeptides B/W) based activation of NPBWR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged NPBWR1 and EA-tagged β-Arrestin-2. Activation of NPBWR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
ATCC 10 STR Profiling Authentication Services
One complete human cell line authentication service, utilizing Short Tanden Repeat (STR) profiling. Trained experts amplify 17 STR loci plus Amelogenin using Promega’s PowerPlex 18D System. A comprehensive analysis report interprets both karyotypically normal and abnormal cell lines, includes a electropherograms supporting the allele calls at each locus, known reference profiling against the ATCC STR database and a comprehensive interpretation of results. This order contains ten sample kits, mailed upon purchase. Sample kit mailed upon purchase. Download the instructions for sample preparation and submission.
